Schematic drawing showing the sorting device and the sorting principle. The cells are introduced into the microchannel through the sample inlet with a volume flow rate Qsample = 20 μl/h and focused to the lower channel wall by sheath flows Qsheath = 180 μl/h, 380 μl/h, and 580 μl/h. While moving along the main channel the cells migrate across the streamlines between x1 and x2. This non-inertial lift leads to different heights of the cells at x2. The broadening of the channel enhances this height difference and enables cell sorting into outlet 1 and outlet 2.
Experimental setup for non-inertial lift induced cell sorting. The syringes are driven by two independent syringe pumps. Videos are recorded to analyze the sorting performance of the device.
Overlays of five consecutive images with a time step dt = 8 ms at x1 (a), x2 (b), and x3 (c) with the corresponding height distributions of MV3-cells and RBCs along the channel. Note the different scale for (c) with respect to (a) and (b). MV3-cells sorted in upper outlet, RBC sorted in lower outlet (Q = 600 μl/h, Hct = 4%, ηext = 7 mPas).
Sorting efficiencies of MV3-melanoma cells at all parameter sets. The trends for the sorting efficiency are clearly visible: increasing sorting efficiency with increasing flow rate and decreasing efficiency with increasing hematocrit.
Sorting efficiency of MV3-cells under various hematocrits and flow rates. In general, it increases with the sheath flow rate and decreases with the hematocrit.
Overview of sorting efficiency, sorting purity, and enrichment of MV3s and RBCs at all measured parameter sets.
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