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Bio-electrospraying of human mesenchymal stem cells: An alternative for tissue engineering
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10.1063/1.4819747
/content/aip/journal/bmf/7/4/10.1063/1.4819747
http://aip.metastore.ingenta.com/content/aip/journal/bmf/7/4/10.1063/1.4819747

Figures

Image of FIG. 1.
FIG. 1.

Bio-electrospraying equipment.

Image of FIG. 2.
FIG. 2.

Influence of bio-electrospraying voltage (a), flow rate (b) and distance (c) on viability of MSC. Number of recovered MSC after BES (d) (n = 4). (*P < 0.05 and P < 0.001 using ANOVA followed by Tukey post-test)

Image of FIG. 3.
FIG. 3.

Influence of bio-electrospraying time on viability of cells. (n = 4) No significant differences were observed using Anova followed by Tukey post test.

Image of FIG. 4.
FIG. 4.

Morphology of bio-electrosprayed mesenchymal stem cells after 1 day of culture: control group (a) and 15 min (b), 30 min (c), and 60 min (d) of BES. Scale bars represent 1 m. The images were taken using 200× magnification.

Image of FIG. 5.
FIG. 5.

MTT assay showing no differences in the metabolism/proliferation between the control group and the bio-electrosprayed MSC after 1, 7, and 15 days in culture. The bio-electrosprayed MSC showed a gradual and significant increase of viable cells over 15 days. (n = 5) (P < 0.001 using two-way ANOVA)

Image of FIG. 6.
FIG. 6.

Chondrogenic, adipogenic and osteogenic differentiation of MSC after staining with specific dyes for each differentiation protocol. The results showed no differences in the plasticity of bio-electrosprayed MSC at any time during analysis, compared to the positive control group (n = 3). Black arrows indicate fat deposits. Scale bars represent 1 m. The pictures were taken using 400× of magnification.

Image of FIG. 7.
FIG. 7.

DNA damage evaluation in MSC after 15, 30, and 60 min of BES (n = 3). Cultured cells for 1 h before performing the comet assay (a), cultured cells for 3 h before performing the comet assay (b) and cultured cells for 5 h before performing the comet assay (c). Comparison between time of culture before performing the comet assay (d). The DI reduction suggests DNA repair. (P < 0,05, **P < 0,01 and ***P < 0,001 using ANOVA followed by Tukey post-test)

Tables

Generic image for table
Table I.

Immunophenotypic profile of bio-electrosprayed MSC (n = 3). Results less than 0.1% were considered 0%.

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/content/aip/journal/bmf/7/4/10.1063/1.4819747
2013-08-29
2014-04-20
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Bio-electrospraying of human mesenchymal stem cells: An alternative for tissue engineering
http://aip.metastore.ingenta.com/content/aip/journal/bmf/7/4/10.1063/1.4819747
10.1063/1.4819747
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