(a) Schematics of a microfluidic system consisting of a stirring microchamber and microchannel. (b) Platelets are sheared and activated by the rotating stirrer. (c) Activated platelets aggregated and adhered on the collagen-coated surface at the microchannel entrance.
(a) A photograph of the microfluidic chip with blood migrating through the microchannels. (b) Normalized migration of blood samples exposed to various shear rates. (c) Migration velocities at various shear rates.
Comparison of migration ratios normalized for migration distance at a fixed time (t = 30 s, MR30) and stopped position (t = final, MR∞). Both of MR showed minimum at 3600 s−1 (or τ = 12.7 Pa). Blood with platelets removed (negative control) does not show any shear-dependence. The coefficients of variation were less than 3.5%.
Measurements of activated platelets at various shear rate with monitoring the expression of CD62-PE and CD41FITC antibody solution. The shear rate was induced by a rotating stirrer in a microchamber and activated platelets were quantified (with Coulter EPICS XL-MCL flow cytometer equipped with a 488 nm laser, Beckman Coulter, USA). The shearing time was 3 min at each shear rate. The coefficients of variation were less than 4.6%.
Morphological changes of erythrocytes with varying shear rates at fixed shearing time (3 min). (a) Shear rate of 900 s−1 (b) 3600 s−1 and (c) 4500 s−1.
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