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Using microfluidic chip to form brain-derived neurotrophic factor concentration gradient for studying neuron axon guidance
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1.
1. Akiyama, H. , Matsu-ura, T. , Mikoshiba, K. , and Kamiguchi, H. , Sci. Signal. 2, ra34 (2009).
http://dx.doi.org/10.1126/scisignal.2000196
2.
2. Brett, M. E. , Deflorio. R. , Stone, D. E. , and Eddington, D. T. , Lab Chip 12, 31273134 (2012).
http://dx.doi.org/10.1039/c2lc40398f
3.
3. Brewer, G. J. , J. Neurosci. Res. 42, 674768 (1995).
http://dx.doi.org/10.1002/jnr.490420510
4.
4. Cheng, P. L. , Song, A. H. , Wong, Y. H. , Wang, S. , Zhang, X. , and Poo, M. M. , Proc. Natl. Acad. Sci. U.S.A. 108, 1843018435 (2011).
http://dx.doi.org/10.1073/pnas.1115907108
5.
5. Chung, B. G. and Choo, J. , Electrophoresis 31, 30143027 (2010).
http://dx.doi.org/10.1002/elps.201000137
6.
6. Chung, C. A. and Chen, C. Y. , J. Theor. Biol. 261, 610625 (2009).
http://dx.doi.org/10.1016/j.jtbi.2009.08.030
7.
7. Dertinger, S. K. W. , Chiu, D. T. , Jeon, N. L. , and Whitesides, G. M. , Anal. Chem. 73, 12401246 (2001).
http://dx.doi.org/10.1021/ac001132d
8.
8. Falasca, M. , Raimondi, C. , and Maffucci, T. , Methods Mol. Biol. 769, 8795 (2011).
http://dx.doi.org/10.1007/978-1-61779-207-6_7
9.
9. Ferrario, J. E. , Baskaran, P. , Clark, C. , Hendry, A. , Lerner, O. , Hintze, M. , Allen, J. , Chilton, J. K. , and Guthrie, S. , Proc. Natl. Acad. Sci. U.S.A. 109, 1466914674 (2012).
http://dx.doi.org/10.1073/pnas.1116481109
10.
10. Forbes, E. M. , Thompson, A. W. , Yuan, J. , and Goodhill, G. J. , Neuron 74, 490503 (2012).
http://dx.doi.org/10.1016/j.neuron.2012.02.035
11.
11. Fuchs, J. , Stettler, O. , Alvarez-Fischer, D. , Prochiantz, A. , Moya, K. L. , and Joshi, R. L. , Eur. J. Neurosci. 35, 18371845 (2012).
http://dx.doi.org/10.1111/j.1460-9568.2012.08139.x
12.
34. Jeon, N. L. , Baskaran, H. , Dertinger, S. K. W. , Whitesides, G. M. , Van de Water, L. , and Toner, M. , Nat. Biotechnol. 20, 826830 (2002).
http://dx.doi.org/10.1038/nbt712
13.
12. Shen, K. and Cowan, C. W. , Cold Spring Harb. Perspect. Biol. 2, a001842 (2010).
http://dx.doi.org/10.1101/cshperspect.a001842
14.
13. Kennedy, T. E. , Wang, H. , Marshall, W. , and Tessier-Lavigne, M. , J. Neurosci. 26, 88668874 (2006).
http://dx.doi.org/10.1523/JNEUROSCI.5191-05.2006
15.
14. Kim, B. J. , Hannanta-anan, P. , Chau, M. , Kim, Y. S. , Swartz, M. A. , and Wu, M. , PLoS One 8, e68422 (2013).
http://dx.doi.org/10.1371/journal.pone.0068422
16.
15. Kim, S. , Kim, H. J. , and Jeon, N. L. , Integr. Biol. 2, 584603 (2010).
http://dx.doi.org/10.1039/c0ib00055h
17.
16. Kim, S. H. , Kang, J. H. , Chung, I. Y. , and Chung, B. G. , Electrophoresis 32, 254260 (2011).
http://dx.doi.org/10.1002/elps.201000501
18.
17. Kothapalli, C. R. , van Veen, E. , de Valence, S. , Chung, S. , Zervantonakis, I. K. , Gertler, F. B. , and Kamm, R. D. , Lab Chip 11, 497507 (2011).
http://dx.doi.org/10.1039/c0lc00240b
19.
18. Lange, J. , Yafai, Y. , Noack, A. , Yang, X. M. , Munk, A. B. , Krohn, S. , Iandiev, I. , Wiedemann, P. , Reichenbach, A. , and Eichler, W. , Glia 60, 15671578 (2012).
http://dx.doi.org/10.1002/glia.22376
20.
19. Li, J. , Zhu, L. , Zhang, M. , and Lin, F. , Biomicrofluidics 6, 24121 (2012).
http://dx.doi.org/10.1063/1.4718721
21.
20. Lindsley, T. A. , Shah, S. N. , and Ruggiero, E. A. , Alcohol Clin. Exp. Res. 35, 13211330 (2011).
http://dx.doi.org/10.1111/j.1530-0277.2011.01468.x
22.
21. Lowery, L. A. and Van Vactor, D. , Nat. Rev. Mol. Cell Biol. 10, 332343 (2009).
http://dx.doi.org/10.1038/nrm2679
23.
22. Masuda, T. , Sakuma, C. , Taniguchi, M. , Kanemoto, A. , Yoshizawa, M. , Satomi, K. , Tanaka, H. , Takeuchi, K. , Ueda, S. , Yaginuma, H. , and Shiga, T. , Brain Res. 1480, 3040 (2012).
http://dx.doi.org/10.1016/j.brainres.2012.08.055
24.
23. Meier, B. , Zielinski, A. , Weber, C. , Arcizet, D. , Youssef, S. , Franosch, T. , Rädler, J. O. , and Heinrich, D. , Proc. Natl. Acad. Sci. U.S.A. 108, 1141711422 (2011).
http://dx.doi.org/10.1073/pnas.1014853108
25.
24. Mortimer, D. , Pujic, Z. , Vaughan, T. , Thompson, A. W. , Feldner, J. , Vetter, I. , and Goodhill, G. J. , Proc. Natl. Acad. Sci. U.S.A. 107, 52025207 (2010).
http://dx.doi.org/10.1073/pnas.0909254107
26.
25. Nitta, N. , Aoki, Y. , Isogawa, Y. , Tsuchiya, T. , Tsuchiya, T. , and Kanegasaki, S. , Eur. J. Cell Biol. 88, 541549 (2009).
http://dx.doi.org/10.1016/j.ejcb.2009.03.004
27.
26. Polacheck, W. J. , Li, R. , Uzel, S. G. M. , and Kamm, R. , Lab Chip 13, 22522267 (2013).
http://dx.doi.org/10.1039/c3lc41393d
28.
27. Raja, W. K. , Gligorijevic, B. , Wyckoff, J. , Condeelis, J. S. , and Castracane, J. , Integr. Biol. 2, 696706 (2010).
http://dx.doi.org/10.1039/c0ib00044b
29.
28. Seidi, A. , Kaji, H. , Annabi, N. , Ostrovidov, S. , Ramalingam, M. , and Khademhosseini, A. , Biomicrofluidics 5, 22214 (2011).
http://dx.doi.org/10.1063/1.3580756
30.
29. Thompson, A. W. , Pujic, Z. , Richards, L. J. , and Goodhill, G. J. , Mol. Cell Neurosci. 47, 4552 (2011).
http://dx.doi.org/10.1016/j.mcn.2011.02.012
31.
30. Tirella, A. , Marano, M. , Vozzi, F. , and Ahluwalia, A. , Toxicol. In Vitro 22, 19571964 (2008).
http://dx.doi.org/10.1016/j.tiv.2008.09.016
32.
31. Togashi, K. , von Schimmelmann, M. J. , Nishiyama, M. , Lim, C. S. , Yoshida, N. , Yun, B. , Molday, R. S. , Goshima, Y. , and Hong, K. , Neuron 58, 694707 (2008).
http://dx.doi.org/10.1016/j.neuron.2008.03.017
33.
32. Tojima, T. , Hines, J. H. , Henley, J. R. , and Kamiguchi, H. , Nat. Rev. Neurosci. 12, 191203 (2011).
http://dx.doi.org/10.1038/nrn2996
34.
33. Walheim, C. C. , Zanin, J. P. , and de Bellard, M. E. , J. Vis. Exp. 59, e3330 (2012).
http://dx.doi.org/10.3791/3330
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/content/aip/journal/bmf/8/1/10.1063/1.4864235
2014-02-19
2015-09-01

Abstract

Molecular gradients play a significant role in regulating biological and pathological processes. Although conventional gradient-generators have been used for studying chemotaxis and axon guidance, there are still many limitations, including the inability to maintain stable tempo-spatial gradients and the lack of the cell monitoring in a real-time manner. To overcome these shortcomings, microfluidic devices have been developed. In this study, we developed a microfluidic gradient device for regulating neuron axon guidance. A microfluidic device enables the generation of Brain-derived neurotrophic factor (BDNF) gradient profiles in a temporal and spatial manner. We test the effect of the gradient profiles on axon guidance, in the BDNF concentration gradient axon towards the high concentration gradient. This microfluidic gradient device could be used as a powerful tool for cell biology research.

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Scitation: Using microfluidic chip to form brain-derived neurotrophic factor concentration gradient for studying neuron axon guidance
http://aip.metastore.ingenta.com/content/aip/journal/bmf/8/1/10.1063/1.4864235
10.1063/1.4864235
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