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Pathogenic Escherichia coli strain discrimination using laser-induced breakdown spectroscopy
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10.1063/1.2752784
/content/aip/journal/jap/102/1/10.1063/1.2752784
http://aip.metastore.ingenta.com/content/aip/journal/jap/102/1/10.1063/1.2752784

Figures

Image of FIG. 1.
FIG. 1.

A LIBS spectrum of E. coli with the relevant atomic, ionic, and molecular emissions identified. The spectrum is dominated by emission from inorganic elements.

Image of FIG. 2.
FIG. 2.

(Color online) DFA plots of LIBS spectra obtained from various strains of E. coli. (a) Canonical discriminant function scores of four strains of E. coli and the TSA nutrient medium on which they were prepared. (b) Canonical discriminant function scores of three E. coli strains (strain HF4714, a K-12 derivative, has been omitted). (c) Canonical discriminant function scores of the TSA nutrient medium and two strains of E. coli: pathogenic EHEC and the environmental strain Nino C. Almost complete discrimination is achieved between these two strains using DF2.

Image of FIG. 3.
FIG. 3.

(Color online) DFA plots of LIBS spectra obtained from E. coli strains HF4714 and Nino C cultured on a TSA plate (open symbols) and in a TSB (liquid) medium (closed symbols). (a) Canonical discriminant function scores of the two strains and the TSA nutrient medium. DF2 scores provided discrimination between strains regardless of what medium the bacteria were cultured in. (b) Canonical discriminant function scores of the two strains alone. DF1 scores provided the discrimination between strains regardless of what medium the bacteria were cultured in. (c) Canonical discriminant function scores of the two strains grouped together regardless of nutrient medium as well as the TSA.

Tables

Generic image for table
Table I.

Resolved spectral emission lines observed in LIBS spectra acquired from Escherichia coli (Strain Nino C), the nutrient-rich growth medium TSA, and the nutrient-free 0.7% agar substrate on which the bacterial samples were ablated. The atomic and molecular lines/bands are identified and categorized by whether they were visible in the bacteria only (1), in the bacteria and TSA (2), or in the bacteria, TSA, and agar (3).

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/content/aip/journal/jap/102/1/10.1063/1.2752784
2007-07-05
2014-04-25
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Pathogenic Escherichia coli strain discrimination using laser-induced breakdown spectroscopy
http://aip.metastore.ingenta.com/content/aip/journal/jap/102/1/10.1063/1.2752784
10.1063/1.2752784
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