(A) A top-down schematic of the nanofluidic device. The nanoslit separation channel is offset from the intersection by to facilitate DNA plug formation and launch. The separation channel is in length depending upon the device. (B) A cross-sectional schematic of the transition of DNA from its three-dimensional relaxed state to its two-dimensional squished state when forced into a nanochannel. In two dimensions, the molecule becomes a pancake-like entity composed of sub-blobs of DNA each with a diameter equal to the channel height.
Examples of three electropherograms showing the separation of DNA mixtures in the deep separation channels. These experiments were performed using applied between reservoirs 2 and 3.
Electropherograms showing no separation of 2 and DNA in deep channels. Other than the difference in the channel depth, experimental conditions are identical to those used in the deep channel experiments. The spikes apparent in the data are from adhered clumps of DNA that happened to release during these experiments.
Experimental mobility data are plotted along with Eq. (5). Error bars are one standard deviation.
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