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Integration of minisolenoids in microfluidic device for magnetic bead–based immunoassays
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10.1063/1.2801347
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    Affiliations:
    1 Ecole Normale Supérieure, 24 Rue Lhomond, 75231 Paris, France, College of Chemistry and Molecular Sciences, Wuhan University, 430072 Wuhan, People’s Republic of China, and State Key Laboratory of Virology, Wuhan University, 430072 Wuhan, People’s Republic of China
    2 Ecole Normale Supérieure, 24 Rue Lhomond, 75231 Paris, France and Department of Physics, Wuhan University, 430072 Wuhan, People’s Republic of China
    3 College of Chemistry and Molecular Sciences, Wuhan University, 430072 Wuhan, People’s Republic of China and State Key Laboratory of Virology, Wuhan University, 430072 Wuhan, People’s Republic of China
    4 Ecole Normale Supérieure, 24 Rue Lhomond, 75231 Paris, France, College of Chemistry and Molecular Sciences, Wuhan University, 430072 Wuhan, People’s Republic of China, and State Key Laboratory of Virology, Wuhan University, 430072 Wuhan, People’s Republic of China
    5 Ecole Normale Supérieure, 24 Rue Lhomond, 75231 Paris, France
    6 College of Chemistry and Molecular Sciences, Wuhan University, 430072 Wuhan, People’s Republic of China and State Key Laboratory of Virology, Wuhan University, 430072 Wuhan, People’s Republic of China
    a) Tel.: 33-1-44322421. FAX: 33-1-44322402. Electronic mail: yong.chen@ens.fr
    b) Tel.: 86-27-68756759. FAX: 86-27-68574067. Electronic mail: dwpang@whu.edu.cn
    J. Appl. Phys. 102, 084911 (2007); http://dx.doi.org/10.1063/1.2801347
/content/aip/journal/jap/102/8/10.1063/1.2801347
http://aip.metastore.ingenta.com/content/aip/journal/jap/102/8/10.1063/1.2801347
View: Figures

Figures

Image of FIG. 1.
FIG. 1.

(Color online) Layout (a) and photograph (b) of an integrated microfluidic device with minisolenoids for superparamagnetic bead–based heterogeneous immunoassay. A schematic diagram of magnetic trapping is also shown (c).

Image of FIG. 2.
FIG. 2.

(Color online) Schematic representation of the experiment protocol for magnetoimmunoassay. (a) switching on the magnetic field and injection of superparamagnetic beads into the microchannel; (b) washing with ; (c) injecting antigens and incubating for ; (d) washing out unbound antigens with ; (e) flowing fluorescence labeled antibody and incubating; (f) fluorescence detection; and (g) switching off the magnetic field and flushing out superparamagnetic beads for next assays.

Image of FIG. 3.
FIG. 3.

(Color online) (A) Three geometrical arrangements of the two minisolenoids: (a) longitudinal mode, (b) transverse mode, and (c) axial mode. The corresponding magnetic field lines are plotted in (d)–(f). (B) Calibration curve of induced magnetic field for the minisolenoids in transverse mode.

Image of FIG. 4.
FIG. 4.

(Color online) (a) Simulation of the induced magnetic field produced by the two minisolenoids. (b) Plot of the magnetic volumetric energy vs the distance in the direction of the channel (longitudinal mode); the hatched zone, symbolically represents the zone of higher gradients. Point A represents the point of highest magnetic field.

Image of FIG. 5.
FIG. 5.

(Color online) (a) Fluorescence image resulting from the immunoassay with antigen. [(b) and (c)] Calibration curve of the goat IgG generated from a sandwich immunoassay. Biotinylated rabbit antigoat IgG immobilized on the surface of superparamagnetic beads, FITC conjugated rabbit antigoat IgG was the detectable agent. The error bars indicate standard deviation of four independent experiments.

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/content/aip/journal/jap/102/8/10.1063/1.2801347
2007-10-31
2014-04-23
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Integration of minisolenoids in microfluidic device for magnetic bead–based immunoassays
http://aip.metastore.ingenta.com/content/aip/journal/jap/102/8/10.1063/1.2801347
10.1063/1.2801347
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