(Color online) Layout (a) and photograph (b) of an integrated microfluidic device with minisolenoids for superparamagnetic bead–based heterogeneous immunoassay. A schematic diagram of magnetic trapping is also shown (c).
(Color online) Schematic representation of the experiment protocol for magnetoimmunoassay. (a) switching on the magnetic field and injection of superparamagnetic beads into the microchannel; (b) washing with ; (c) injecting antigens and incubating for ; (d) washing out unbound antigens with ; (e) flowing fluorescence labeled antibody and incubating; (f) fluorescence detection; and (g) switching off the magnetic field and flushing out superparamagnetic beads for next assays.
(Color online) (A) Three geometrical arrangements of the two minisolenoids: (a) longitudinal mode, (b) transverse mode, and (c) axial mode. The corresponding magnetic field lines are plotted in (d)–(f). (B) Calibration curve of induced magnetic field for the minisolenoids in transverse mode.
(Color online) (a) Simulation of the induced magnetic field produced by the two minisolenoids. (b) Plot of the magnetic volumetric energy vs the distance in the direction of the channel (longitudinal mode); the hatched zone, symbolically represents the zone of higher gradients. Point A represents the point of highest magnetic field.
(Color online) (a) Fluorescence image resulting from the immunoassay with antigen. [(b) and (c)] Calibration curve of the goat IgG generated from a sandwich immunoassay. Biotinylated rabbit antigoat IgG immobilized on the surface of superparamagnetic beads, FITC conjugated rabbit antigoat IgG was the detectable agent. The error bars indicate standard deviation of four independent experiments.
Article metrics loading...
Full text loading...