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Photobleaching-free infrared near-field microscopy localizes molecules in neurons
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View: Figures


Image of FIG. 1.
FIG. 1.

(a) Scheme of the labeling reaction used; the star indicates the label, which is the fluorophore Alexa 488. (b) Confocal microscopy image obtained after labeling. (c) FTIR spectra of the (reference) neuron cells not transfected with the fluorophore (dashed line) and of the die Alexa 488 (solid line). Note that the two curves are raw data, not normalized (since the concentration of the die in the neuron is unknown): a direct comparison between the intensities of the two curves is therefore impossible.

Image of FIG. 2.
FIG. 2.

(a) topographical shear-force image of a neuron cell. A dashed white line delineates the borders of the cell. White spots are protrusions while dark areas are valleys. (b) Absorption map at , the absorption peak of the Alexa 488 fluorophore. A cutoff filter was used in this case to enhance the absorption contrast. The dark (high absorption) spots correspond to the Alexa 488 molecules, attached to the AMPA-GluR2 surface receptors. (c) topographical shear-force image of a neuron cell and (d) corresponding fluorophore absorption map with no cutoff filtering map.

Image of FIG. 3.
FIG. 3.

Set of images on a control neuron cell prepared without the labeling reaction with Alexa 488. (a) Shear-force topography. (b) Absorption map at with a dark feature due to the amide I group absorption. (c) Absorption map at : no features are visible in the absence of Alexa 488.


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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Photobleaching-free infrared near-field microscopy localizes molecules in neurons