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Ultrafast optics: Imaging and manipulating biological systems
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View: Figures


Image of FIG. 1.
FIG. 1.

Induced polarization of molecules outside of (left) and inside of (right) the focal plane of a focusing ultrafast excitation beam.

Image of FIG. 2.
FIG. 2.

General nonlinear microscope layout. Dashed lines represent telecentric planes in scanning optics system.

Image of FIG. 3.
FIG. 3.

Telecentric system to translate an angular deflection of the excitation beam into a lateral translation of of the focal spot in the sample.

Image of FIG. 4.
FIG. 4.

(a) Schematic of 2D SI apparatus and (b) a sample SI. The curvature of the fringes in this case is indicative of the presence of spherical aberration.

Image of FIG. 5.
FIG. 5.

Jablonski diagram showing four common nonlinear imaging modalities. Dotted levels represent virtual energy states.

Image of FIG. 6.
FIG. 6.

TPEF images of YFP labeled corn plant. Images are of the same field of view but taken at different depths in the sample. Relative focal offset between (a) and (b) is .

Image of FIG. 7.
FIG. 7.

Axial stack of SHG images of fixed trigeminal nerve dissected from an adult mouse. Axis labels shown in (a) are the same for (b) through (g). Images are of the same field of view but taken at different depths in the sample. Relative focal offset between subsequent images is .

Image of FIG. 8.
FIG. 8.

Time series of three THG images of cytoplasm streaming within the rhizoid taken at exposure, imaged to a CCD camera. Time between frames is .

Image of FIG. 9.
FIG. 9.

(a) Single frequency CARS and (b) multiplex CARS with longer duration (picosecond) pulses used for the pump and probe beams and short duration (femtosecond) pulses used for the Stokes beam.

Image of FIG. 10.
FIG. 10.

(a) Energy diagram for STED process. (b) Graphic depiction of the depletion of the outer portion of the diffraction-limited spot (shaded circle) by the doughnut-shaped STED spot (dashed circles).

Image of FIG. 11.
FIG. 11.

Simultaneously acquired SHG images of crystalline cellulose at two orthogonal excitation polarizations. Incident electric field is oriented horizontally in (a) and vertically in (b).


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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Ultrafast optics: Imaging and manipulating biological systems