Induced polarization of molecules outside of (left) and inside of (right) the focal plane of a focusing ultrafast excitation beam.
General nonlinear microscope layout. Dashed lines represent telecentric planes in scanning optics system.
Telecentric system to translate an angular deflection of the excitation beam into a lateral translation of of the focal spot in the sample.
(a) Schematic of 2D SI apparatus and (b) a sample SI. The curvature of the fringes in this case is indicative of the presence of spherical aberration.
Jablonski diagram showing four common nonlinear imaging modalities. Dotted levels represent virtual energy states.
TPEF images of YFP labeled corn plant. Images are of the same field of view but taken at different depths in the sample. Relative focal offset between (a) and (b) is .
Axial stack of SHG images of fixed trigeminal nerve dissected from an adult mouse. Axis labels shown in (a) are the same for (b) through (g). Images are of the same field of view but taken at different depths in the sample. Relative focal offset between subsequent images is .
Time series of three THG images of cytoplasm streaming within the rhizoid taken at exposure, imaged to a CCD camera. Time between frames is .
(a) Single frequency CARS and (b) multiplex CARS with longer duration (picosecond) pulses used for the pump and probe beams and short duration (femtosecond) pulses used for the Stokes beam.
(a) Energy diagram for STED process. (b) Graphic depiction of the depletion of the outer portion of the diffraction-limited spot (shaded circle) by the doughnut-shaped STED spot (dashed circles).
Simultaneously acquired SHG images of crystalline cellulose at two orthogonal excitation polarizations. Incident electric field is oriented horizontally in (a) and vertically in (b).
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