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Label-free detection of oligonucleotide microarrays by oblique-incidence reflectivity difference method
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10.1063/1.3327447
/content/aip/journal/jap/107/6/10.1063/1.3327447
http://aip.metastore.ingenta.com/content/aip/journal/jap/107/6/10.1063/1.3327447
View: Figures

Figures

Image of FIG. 1.
FIG. 1.

Sketch of OI-RD system for the detection of oligonucleotide microarrays. The microarray is mounted on a motorized stage that can be driven along and direction, respectively. Laser: He–Ne polarized laser; PEM: Photoelastic modulator.

Image of FIG. 2.
FIG. 2.

(a) image of labeled microarray M1 after hybridization. (b) Fluorescence image of labeled microarray M1 after hybridization. Probe-I area is in the left, Probe-II area is in the right. (c) Left image: Gray scale image of for Probe-I and Probe-II averaged from each three columns. Right curves: and fluorescence intensities over the printed triplicate spots area vs the concentration.

Image of FIG. 3.
FIG. 3.

Top image is the two-dimensional scan image of for unlabeled microarray M2 after hybridization. Bottom curve is the intensity profile taken along the dotted line in the top image.

Image of FIG. 4.
FIG. 4.

Gray scale image drew with averaged over every sample area from top image in Fig. 3. The bottom is the further averaged value of the top three rows.

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/content/aip/journal/jap/107/6/10.1063/1.3327447
2010-03-30
2014-04-18
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Label-free detection of oligonucleotide microarrays by oblique-incidence reflectivity difference method
http://aip.metastore.ingenta.com/content/aip/journal/jap/107/6/10.1063/1.3327447
10.1063/1.3327447
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