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Immunomagnetic reduction assay using high- superconducting-quantum-interference-device-based magnetosusceptometry
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10.1063/1.3340861
/content/aip/journal/jap/107/7/10.1063/1.3340861
http://aip.metastore.ingenta.com/content/aip/journal/jap/107/7/10.1063/1.3340861

Figures

Image of FIG. 1.
FIG. 1.

Instruction of physical mechanism of IMR assay. (a) The ac susceptibility generated with the individual biofunctionalized magnetic nanoparticles in magnetic reagent, and (b) the ac susceptibility of the magnetic reagent after the association between biofunctionalized magnetic nanoparticles and biotargets.

Image of FIG. 2.
FIG. 2.

Scheme of the four-channel SQUID-based ac magnetosuscptometry.

Image of FIG. 3.
FIG. 3.

Frequency dependent shielding factor of the magnetically shielded box is shape of cylinder used in the SQUID-based magnetosusceptometry shown in Fig. 2.

Image of FIG. 4.
FIG. 4.

Real-time signals (i.e., curve) of four identical magnetic reagents mixed with four identical 100 ng/ml NEGF solution. Each curve is probed with one channel of the four-channel SQUID-based magnetosusceptometry shown in Fig. 2.

Image of FIG. 5.
FIG. 5.

IMR signal as a function of the concentration of VEGF . The data points are fitted to logistic function expressed as Eq. (3).

Image of FIG. 6.
FIG. 6.

Scaling feature for the characteristic curves of IMR assays on proteins (VEGF), viruses (H5N1), and cytokines .

Tables

Generic image for table
Table I.

Resistance and inductance of excitation coils and pick-up coil used in the multichannel SQUID-based magneosusceptometry schematically shown in Fig. 2.

Generic image for table
Table II.

Detected mean value of concentration , standard deviation of measured concentration , and the CV from triple tests, and the error (%) via Eq. (4) for each spiked concentration .

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/content/aip/journal/jap/107/7/10.1063/1.3340861
2010-04-15
2014-04-16
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Immunomagnetic reduction assay using high-Tc superconducting-quantum-interference-device-based magnetosusceptometry
http://aip.metastore.ingenta.com/content/aip/journal/jap/107/7/10.1063/1.3340861
10.1063/1.3340861
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