Experimental circuit for the measurement of ac susceptibility of magnetic markers in solution.
Size distribution of magnetic markers. The curve was measured with DLS. The curve was obtained by analyzing the frequency dependence of susceptibility shown in Fig. 3 using the SVD method.
Frequency dependence of the complex susceptibility of magnetic markers in solution. and represent the real and imaginary parts, respectively. The symbols represent experimental results, while the solid lines are calculated from Eqs. (1) and (2).
Relationship between the magnetization and the amplitude of the excitation field . The symbols represent experimental results, while the solid lines were calculated from the fundamental component of the Langevin function .
Relationship between magnetization and weight of the magnetic markers. The imaginary part was measured at and .
Detection principle of liquid phase immunoassay. Markers were bound to the targets that were fixed on the surface of large polymer beads. The difference between the Brownian relaxation time of the bound and free markers was used.
Change in susceptibility when avidin-coated markers were bound to biotin-conjugated polymer beads. (a) real part and (b) imaginary part.
Relationship between the number of bound markers and the number of biotin-conjugated polymer beads. is the ratio of to the total number of markers. The results for different incubation times are shown.
Binding process between avidin-coated markers and biotin-conjugated polymer beads. Change in with time after the polymer beads and markers were put into the solution are shown for the cases of , , and . The solid lines were calculated from Eq. (6).
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