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ac susceptibility measurement of magnetic markers in suspension for liquid phase immunoassay
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10.1063/1.3457233
/content/aip/journal/jap/108/3/10.1063/1.3457233
http://aip.metastore.ingenta.com/content/aip/journal/jap/108/3/10.1063/1.3457233
View: Figures

Figures

Image of FIG. 1.
FIG. 1.

Experimental circuit for the measurement of ac susceptibility of magnetic markers in solution.

Image of FIG. 2.
FIG. 2.

Size distribution of magnetic markers. The curve was measured with DLS. The curve was obtained by analyzing the frequency dependence of susceptibility shown in Fig. 3 using the SVD method.

Image of FIG. 3.
FIG. 3.

Frequency dependence of the complex susceptibility of magnetic markers in solution. and represent the real and imaginary parts, respectively. The symbols represent experimental results, while the solid lines are calculated from Eqs. (1) and (2).

Image of FIG. 4.
FIG. 4.

Relationship between the magnetization and the amplitude of the excitation field . The symbols represent experimental results, while the solid lines were calculated from the fundamental component of the Langevin function .

Image of FIG. 5.
FIG. 5.

Relationship between magnetization and weight of the magnetic markers. The imaginary part was measured at and .

Image of FIG. 6.
FIG. 6.

Detection principle of liquid phase immunoassay. Markers were bound to the targets that were fixed on the surface of large polymer beads. The difference between the Brownian relaxation time of the bound and free markers was used.

Image of FIG. 7.
FIG. 7.

Change in susceptibility when avidin-coated markers were bound to biotin-conjugated polymer beads. (a) real part and (b) imaginary part.

Image of FIG. 8.
FIG. 8.

Relationship between the number of bound markers and the number of biotin-conjugated polymer beads. is the ratio of to the total number of markers. The results for different incubation times are shown.

Image of FIG. 9.
FIG. 9.

Binding process between avidin-coated markers and biotin-conjugated polymer beads. Change in with time after the polymer beads and markers were put into the solution are shown for the cases of , , and . The solid lines were calculated from Eq. (6).

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/content/aip/journal/jap/108/3/10.1063/1.3457233
2010-08-05
2014-04-16
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: ac susceptibility measurement of magnetic markers in suspension for liquid phase immunoassay
http://aip.metastore.ingenta.com/content/aip/journal/jap/108/3/10.1063/1.3457233
10.1063/1.3457233
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