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Static and dynamic observation of supermolecular protein, ferritin, using high-speed atomic force microscope
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10.1063/1.3544011
/content/aip/journal/jap/109/3/10.1063/1.3544011
http://aip.metastore.ingenta.com/content/aip/journal/jap/109/3/10.1063/1.3544011
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Figures

Image of FIG. 1.
FIG. 1.

Structure of ferritin molecule. (a) Appearance of ferritin and (b) Cross section model of ferritin core. The spherical hollow shell is composed of 24 polypeptide subunits. The inner and outer diameters of the protein shell are about 7 nm and 13 nm, respectively. The self-assembled protein cage has the ability to sequester and store iron as hydrated iron oxide in the internal cavity.

Image of FIG. 2.
FIG. 2.

Two-dimensional ferritin crystal array with field emission type high resolution SEM. The white dots represent ferritin cores. The outer protein shell was removed by UV ozone treatment. The ferritin molecule densities of the core were approximately (a) , (b) , and (c) , respectively.

Image of FIG. 3.
FIG. 3.

Difference in amounts of ferritin adsorbed between hydrophilic and hydrophobic substrate surfaces. (a) Hydrophilic, (b) slightly hydrophilic, (c) slightly hydrophobic, and (d) coexisting hydrophilic and hydrophobic areas. The dots were arranged randomly. Some dots gathered to form clusters, and some dots were isolated.

Image of FIG. 4.
FIG. 4.

Machining of ferritin protein molecule in pure water using a conventional AFM. (a) Before machining and (b) after machining with a force of 700 nN.

Image of FIG. 5.
FIG. 5.

Dynamic images of ferritin molecule-substrate interaction in pure water by high-speed AFM. (a) First image before adding ferritin solution. [(b)–(n)] Dynamic images of ferritin molecules after adding ferritin solution. The arrows from (b) to (n) show the same molecule of ferritin. (o) High-speed AFM image of a ferritin indicated by the arrow in figure and (p) cross-sectional profile of (o).

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/content/aip/journal/jap/109/3/10.1063/1.3544011
2011-02-02
2014-04-16
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Static and dynamic observation of supermolecular protein, ferritin, using high-speed atomic force microscope
http://aip.metastore.ingenta.com/content/aip/journal/jap/109/3/10.1063/1.3544011
10.1063/1.3544011
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