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Alternating current impedance spectroscopic analysis of biofunctionalized vertically-aligned silica nanospring surface for biosensor applications
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10.1063/1.3601521
/content/aip/journal/jap/110/1/10.1063/1.3601521
http://aip.metastore.ingenta.com/content/aip/journal/jap/110/1/10.1063/1.3601521

Figures

Image of FIG. 1.
FIG. 1.

(Color online) Scanning electron microscopy (SEM) images of (a) top view of the VANS, (b) side view of the VANS, and (c) a VANS sensor mounted on the test jig used in this study.

Image of FIG. 2.
FIG. 2.

(Color online) A schematic of protein immobilization on the surface of the VANS.

Image of FIG. 3.
FIG. 3.

(Color online) Impedance spectra measured at 10 mV applied Vrms after the subsequent addition of B-IgG (or IgG), Av-GOx, and B-BSA-FITC: (a) impedance magnitude plot, (b) impedance phase plot as a function of frequency of the ITO sensor, (c) impedance magnitude plot, and (d) impedance phase plot as a function of frequency of the control (ITO sensor with IgG).

Image of FIG. 4.
FIG. 4.

(Color online) Histograms of fluorescence emission of B-BSA-FITC: (a) intensity histogram of ITO sensor with B-IgG, (b) intensity histogram of the control (ITO sensor with IgG), (c) intensity histogram of VANS sensor with B-IgG, and (d) intensity histogram of the control (VANS sensor with IgG).

Image of FIG. 5.
FIG. 5.

(a) The equivalent circuit of the ITO sensor and (b) the equivalent circuit of the VANS sensor.

Image of FIG. 6.
FIG. 6.

(Color online) (a) Measured and fitted impedance magnitude plot, (b) measured and fitted impedance phase plot as a function of frequency of the ITO sensor, (c) measured and fitted impedance magnitude plot, and (d) measured and fitted impedance phase plot as a function of frequency of the VANS sensor.

Image of FIG. 7.
FIG. 7.

(Color online) Impedance spectra measured at 10 mV applied Vrms after the subsequent addition of B-IgG (or IgG), Av-GOx, and B-BSA-FITC: (a) impedance magnitude plot, (b) impedance phase plot as a function of frequency of the VANS sensor with B-IgG, (c) impedance magnitude plot, and (d) impedance phase plot as a function of frequency of the control (VANS sensor with IgG).

Tables

Generic image for table
Table I.

Electrical element values in the equivalent circuit of the ITO sensor shown in Fig. 5(a) as a function of B-IgG, Av-GOx, and B-BSA-FITC, as obtained from the fitting of the impedance and phase spectra.

Generic image for table
Table II.

Average values of the circuit elements in the equivalent circuit shown in Fig. 5(b) obtained from fitting the experimental impedance spectra as a function of B-IgG, Av-GOx, and B-BSA-FITC for the VANS sensor.

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/content/aip/journal/jap/110/1/10.1063/1.3601521
2011-07-01
2014-04-18
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Alternating current impedance spectroscopic analysis of biofunctionalized vertically-aligned silica nanospring surface for biosensor applications
http://aip.metastore.ingenta.com/content/aip/journal/jap/110/1/10.1063/1.3601521
10.1063/1.3601521
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