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Effects of cell cycle on the uptake of water soluble quantum dots by cells
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10.1063/1.3669364
/content/aip/journal/jap/110/12/10.1063/1.3669364
http://aip.metastore.ingenta.com/content/aip/journal/jap/110/12/10.1063/1.3669364

Figures

Image of FIG. 1.
FIG. 1.

(Color online) Temperature-dependent cellular uptake of QDs studied by LSCM. The Hela cells were incubated with thiol-capped CdTe QDs (0.5 μM) for 1 h at 4 °C (upper) and 37 °C (lower). Left: PL images; middle: DIC images; right: merged images.

Image of FIG. 2.
FIG. 2.

(Color online) Temperature-dependent cellular uptake of AlPcSs as a control of the typical endocytosis. The Hela cells were incubated with AlPcSs (6 μM) for 1 h at 4 °C (upper) and 37 °C (lower). Left: fluorescence images; middle: DIC images; right: merged images.

Image of FIG. 3.
FIG. 3.

(Color online) Effects of cell cycle on the uptake of QDs by cells. (A) Hela cells were synchronized to S phase (98%) with thymidine, (B) Hela cells synchronized to G2/M phase (76%) with nocodazole, (C) 293T cells synchronized to S phase (98%) with thymidine, and (D) 293T cells synchronized to G2/M phase (52%) with nocodazole. For details see II Cells were incubated with QDs (0.5 μM) for 1 h at 37 °C, followed by imaging with LSCM.

Image of FIG. 4.
FIG. 4.

(Color online) Effects of various phases of cell cycle on the uptake of CdTe QDs by three cell lines were measured by flow cytometry. The cells were incubated with CdTe QDs (0.5 μM) for 1 h at 37 °C. The control cell group was not synchronized, neither incubated with CdTe QDs. The x-axis represents the relative fluorescence intensity of the cellular QD; while the y-axis indicates the number of cells. (A), (B), and (C) are the Hela, QGY, and 293T cell lines, respectively.

Tables

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Table I.

Percentages of cell populations in various phases of cell cycle after phase arresting treatments with pharmacological agents measured by flow cytometry.

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Table II.

Relative QD uptake rates of cells in G1, S, and G2/M phases in the three cell lines (the uptake rates in the G1 phase are normalized to 1).

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/content/aip/journal/jap/110/12/10.1063/1.3669364
2011-12-19
2014-04-25
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Effects of cell cycle on the uptake of water soluble quantum dots by cells
http://aip.metastore.ingenta.com/content/aip/journal/jap/110/12/10.1063/1.3669364
10.1063/1.3669364
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