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Mode of bindings of zinc oxide nanoparticles to myoglobin and horseradish peroxidase: A spectroscopic investigations
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10.1063/1.3610446
/content/aip/journal/jap/110/2/10.1063/1.3610446
http://aip.metastore.ingenta.com/content/aip/journal/jap/110/2/10.1063/1.3610446
View: Figures

Figures

Image of FIG. 1.
FIG. 1.

(Color online) (a) Molecular structure of HMb; (b) Molecular structure of HRP; c: HRTEM picture of ZnO nanoparticles.

Image of FIG. 2.
FIG. 2.

(a) UV-vis absorption spectra of HMb (Conc. ∼ 3 × 10−5 M) in presence of ZnO nanoparticles at ambient temperature in buffer solution (pH = 7). The concentrations of ZnO in (0) 0, (1) 7.7 × 10−6 M, (2) 1.54 × 10−5 M, (3) 2.3 × 10−5 M, (4) 3.1 × 10−5 M, (5) 3.8 × 10−5 M, (6) 4.55 × 10−5 M, and (7) 5.29 × 10−5 M. (inset) Magnified version of the Soret band of HMb in absence and presence of ZnO nanoparticle. (b) UV-vis absorption spectra of HRP (Conc. ∼ 2 × 10−5 M) in presence of ZnO nanoparticles at ambient temperature in buffer solution (pH = 7). The concentrations of ZnO in (0) 0, (1) 7.7 × 10−6 M, (2) 1.54 × 10−5M, (3) 2.3 × 10−5 M, (4) 3.1 × 10−5 M, (5) 3.8 × 10−5 M, (6) 4.55 × 10−5 M, and (7) 5.29 × 10−5 M. (top inset) UV-vis absorption spectra of ZnO nanoparticle (Conc. ∼ 5.29 × 10−5 M). (bottom inset) Magnified version of the Soret band of HRP in absence and presence of ZnO nanoparticle.

Image of FIG. 3.
FIG. 3.

(a) Fluorescence emission spectra of HMb (Conc. ∼ 3 × 10−5 M) (λ ex ∼ 295 nm) in presence of ZnO nanoparticles at ambient temperature in buffer solution. The concentrations of ZnO in (0) 0, (1) 7.7 × 10−6 M, (2) 1.54 × 10−5M, (3) 2.3 × 10−5 M, (4) 3.1 × 10−5 M, (5) 3.8 × 10−5 M, (6) 4.55 × 10−5 M, and (7) 5.29 × 10−5 M. (b) Fluorescence emission spectra of HRP (Conc. ∼ 2 × 10−5 M) (λ ex ∼ 295 nm) in presence of ZnO nanoparticles at ambient temperature in buffer solution. The concentrations of ZnO in (0) 0, (1) 7.7 × 10−6 M, (2) 1.54 × 10−5M, (3) 2.3 × 10−5 M, (4) 3.1 × 10−5 M, (5) 3.8 × 10−5 M, (6) 4.55 × 10−5 M, and (7) 5.29 × 10−5 M.

Image of FIG. 4.
FIG. 4.

(a) S-V plot in case of HMb–ZnO nanomaterial complex at three different temperatures; (b) S-V plot in case of HRP–ZnO nanomaterial complex at three different temperatures.

Image of FIG. 5.
FIG. 5.

(a) RLS spectra of (1) HMb (Conc. ∼ 3 × 10−5 M) (2) HMb –ZnO complex. Conc. of ZnO nanoparticles ∼ 5.29 × 10−5 M; (b) RLS spectra of (1) HRP (Conc. ∼ 2 × 10−5 M) (2) HRP –ZnO complex. Conc. of ZnO nanoparticles ∼ 5.29 × 10−5 M.

Image of FIG. 6.
FIG. 6.

(a) FT-IR spectra of HMb (Conc. ∼ 1.7 × 10−2 M) (solid), HMb –ZnO complex (dotted). Conc. of ZnO nanoparticles 7.2 × 10−3 M; (b) FT-IR spectra of HRP (Conc. ∼ 2.0 × 10−2 M) (solid), HRP –ZnO complex (dotted). Conc. of ZnO nanoparticles 7.2 × 10−3 M.

Image of FIG. 7.
FIG. 7.

(a) The synchronous fluorescence spectra of the HMb (Conc. ∼ 3 × 10−5 M) in presence of ZnO nanoparticles where Δλ ∼ 60 nm. The concentration of ZnO nanoparticles in (0) 0, (1) 5.29 × 10−5 M; (b) The synchronous fluorescence spectra of the HMb (Conc. ∼3 × 10−5 M) in presence of ZnO nanoparticles where Δλ ∼ 15 nm. The concentration of ZnO nanoparticles in (0) 0, (1) 5.29 × 10−5 M

Image of FIG. 8.
FIG. 8.

(a) The synchronous fluorescence spectra of the HRP (Conc. ∼ 2 × 10−5 M) in presence of ZnO nanoparticles where Δλ ∼ 60 nm. The concentration of ZnO nanoparticles in (0) 0, (1) 5.29 × 10−5 M; (b) The synchronous fluorescence spectra of the HRP (Conc. ∼ 2 × 10−5 M) in presence of ZnO nanoparticles where Δλ ∼ 15 nm. The concentration of ZnO nanoparticles in (0) 0, (1) 5.29 × 10−5 M.

Image of FIG. 9.
FIG. 9.

(a) CD spectra of HMb (Conc. ∼ 3 × 10−5 M) in presence of ZnO nanoparticles (0) 0, (1) 3.1 × 10−5 M, (2) 3 × 10−4; (b) CD spectra of HMb-ZnO complex at three different temperatures (291, 298, 308 K); (c) CD spectra of HRP (Conc. ∼ 2 × 10−5 M) in presence of ZnO nanoparticles (0) 0, (1) 3.1 × 10−5 M, (2) 2 × 10−4 M; (d) CD spectra of HRP-ZnO complex at three different temperatures (291, 298, 308 K).

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/content/aip/journal/jap/110/2/10.1063/1.3610446
2011-07-29
2014-04-18
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Mode of bindings of zinc oxide nanoparticles to myoglobin and horseradish peroxidase: A spectroscopic investigations
http://aip.metastore.ingenta.com/content/aip/journal/jap/110/2/10.1063/1.3610446
10.1063/1.3610446
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