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The effects of thermal stimuli on intracellular calcium change and histamine releases in rat basophilic leukemia mast cells
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10.1063/1.4723649
/content/aip/journal/jap/111/10/10.1063/1.4723649
http://aip.metastore.ingenta.com/content/aip/journal/jap/111/10/10.1063/1.4723649
View: Figures

Figures

Image of FIG. 1.
FIG. 1.

(a) Scheme of cell dish used for thermal stimulus. (b) The temperature-heating time curve when the cell dish was under heating.

Image of FIG. 2.
FIG. 2.

Sequential Ca2+ fluorescence images of the RBL-2H3 cells in Hank’s buffer under the thermal stimulus. Cells have been stained with fluo-3/AM for Ca2+ detection.

Image of FIG. 3.
FIG. 3.

Temperature dependent profile of Ca2+ fluorescence intensities in mast cells in different extracellular buffer under the thermal stimulus. The “Hank’s” group means the extracellular buffer was Hank’s buffer, and the “RR block” group means that the 12 μM RR has been added in Hank’s buffer. The “saline” group means that extracellular buffer was calcium-free saline.

Image of FIG. 4.
FIG. 4.

(a) The effect of thermal stimulus on histamine release of mast cells in Hank’s buffer. The fluorescence intensities of the histamine-OPA complexes were measure in each group after the cells have been treated at temperatures of 18, 37, 43, 53, and 58 °C for 5 min, respectively. (b) The statistic analysis of thermal stimulus on histamine release of mast cells. The 12 μM RR has been added in the Hank’s buffer of cell dishes and termed as “RR block” group.

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/content/aip/journal/jap/111/10/10.1063/1.4723649
2012-05-30
2014-04-18
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: The effects of thermal stimuli on intracellular calcium change and histamine releases in rat basophilic leukemia mast cells
http://aip.metastore.ingenta.com/content/aip/journal/jap/111/10/10.1063/1.4723649
10.1063/1.4723649
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