(a) Scheme of cell dish used for thermal stimulus. (b) The temperature-heating time curve when the cell dish was under heating.
Sequential Ca2+ fluorescence images of the RBL-2H3 cells in Hank’s buffer under the thermal stimulus. Cells have been stained with fluo-3/AM for Ca2+ detection.
Temperature dependent profile of Ca2+ fluorescence intensities in mast cells in different extracellular buffer under the thermal stimulus. The “Hank’s” group means the extracellular buffer was Hank’s buffer, and the “RR block” group means that the 12 μM RR has been added in Hank’s buffer. The “saline” group means that extracellular buffer was calcium-free saline.
(a) The effect of thermal stimulus on histamine release of mast cells in Hank’s buffer. The fluorescence intensities of the histamine-OPA complexes were measure in each group after the cells have been treated at temperatures of 18, 37, 43, 53, and 58 °C for 5 min, respectively. (b) The statistic analysis of thermal stimulus on histamine release of mast cells. The 12 μM RR has been added in the Hank’s buffer of cell dishes and termed as “RR block” group.
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