1887
banner image
No data available.
Please log in to see this content.
You have no subscription access to this content.
No metrics data to plot.
The attempt to load metrics for this article has failed.
The attempt to plot a graph for these metrics has failed.
Single-cell adhesion probed in-situ using optical tweezers: A case study with Saccharomyces cerevisiae
Rent:
Rent this article for
USD
10.1063/1.4723566
/content/aip/journal/jap/111/11/10.1063/1.4723566
http://aip.metastore.ingenta.com/content/aip/journal/jap/111/11/10.1063/1.4723566

Figures

Image of FIG. 1.
FIG. 1.

(a) Simplified schematic layout of the optical tweezers system. (b) Sketch showing the trapped bead (radius r) with respect to the surface at height h. (c) Ray-tracing representation of the refracted trapping beam, showing a shift between the marginal rays and the paraxial rays, which is responsible for an elongated focal volume (spherical aberration).

Image of FIG. 2.
FIG. 2.

Variation of the apparent drag force as a function of the distance to the wall, as deduced from measurements performed after calibrating the trap force at a distance of 20 μm from the wall, with (solid lines, closed symbols) or without (dashed lines, open symbols) correction for the hydrodynamic wall effect, at different laser powers in the sample.

Image of FIG. 3.
FIG. 3.

Drag force correction for yeast cells as function of the distance to the wall, obtained by using Eq. (9) (solid line) and by CFD (sphere, open circles; prolate spheroid, solid triangles). Inset: relative difference between CFD results and results obtained using Eq. (9).

Image of FIG. 4.
FIG. 4.

(a) Wall-corrected drag force (pN) vs. laser power at the objective, measured at a distance of 3 μm (red closed circles) and 20 μm (black closed squares) from the surface. Experimental data taken at 20 μm and converted to the distance of 3 μm (open circles) though multiplication by β. (b) Conversion factor β described by Eq. (13).

Image of FIG. 5.
FIG. 5.

Micrographs showing budding of a cell in the trap: time zero, Tin situ  = 30.1 °C (a); after 3 h, Tin situ  = 30.3 °C, before (b) and after release and trapping again (c); after 5 h, Tin situ  = 30.2 °C, before (d) and after release and trapping again (e). Scale bar, 5 μm.

Image of FIG. 6.
FIG. 6.

Zeta potential (mV) as a function of ionic strength (mM) for (a) the glass particles and (b) the yeast cells, in the presence of NaCl (open symbols) or CaCl2 (closed symbols). Dashed lines are decaying exponential fits.

Image of FIG. 7.
FIG. 7.

Proportion of firmly, mildly, and non-adhering cells (%) ratioed to the total population, measured at 1 h contact time, at different CaCl2 IS (50, 80, 100, 150, 200, and 330 mM) and NaCl IS (150, 180, 200, 250, and 330 mM).

Image of FIG. 8.
FIG. 8.

Proportion of mildly adhering cells (in a.u., normalized by the maximum value) removed after 1 h contact time for different intervals of the applied force (pN) at different CaCl2 IS (50, 80, 100, 150, 200, and 330 mM) and NaCl IS (150, 180, 200, and 250 mM). Inset: plots of the median detachment forces F* (pN) vs. (mM0.5) with Ca and Na with linear fits (solid lines).

Image of FIG. 9.
FIG. 9.

Potential energy (left axis, 103 kT, solid line) and force (right axis, 103 pN, dashed line) computed by the DLVO theory as function of separation distance (nm) in NaCl 150 mM solution. Inset is a close-up of the figure.

Tables

Generic image for table
Table I.

Values (mean ± standard deviation (SD)) of yeast cell dimensions a and b of related parameters and of shape-drag correction factor Ks .

Generic image for table
Table II.

Force level toward the barrier (nN), energy barrier (kT) and force level towards the secondary minimum (pN) as function of IS. nb, no barrier.

Generic image for table
Table III.

Comparison of single-cell techniques (modified from Ref. 68).

Loading

Article metrics loading...

/content/aip/journal/jap/111/11/10.1063/1.4723566
2012-06-01
2014-04-21
Loading

Full text loading...

This is a required field
Please enter a valid email address
752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Single-cell adhesion probed in-situ using optical tweezers: A case study with Saccharomyces cerevisiae
http://aip.metastore.ingenta.com/content/aip/journal/jap/111/11/10.1063/1.4723566
10.1063/1.4723566
SEARCH_EXPAND_ITEM