Skip to main content
banner image
No data available.
Please log in to see this content.
You have no subscription access to this content.
No metrics data to plot.
The attempt to load metrics for this article has failed.
The attempt to plot a graph for these metrics has failed.
The full text of this article is not currently available.
1. K. I. Draget and C. Taylor, “ Chemical, physical and biological properties of alginates and their biomedical implications,” Food Hydrocolloids 25(2), 251256 (2011).
2. K. Y. Lee and D. J. Mooney, “ Alginate: properties and biomedical applications,” Prog. Polym. Sci. 37(1), 106126 (2012).
3. K. I. Draget, “ Alginates,” in Handbook of Hydrocolloids, 2nd ed., edited by G. O. Phillips and P. A. Williams ( Woodhead Publishing, 2009), pp. 807828.
4. S.-J. Shin, J.-Y. Park, J.-Y. Lee, H. Park, Y.-D. Park, K.-B. Lee, C.-M. Whang, and S.-H. Lee, “‘ On the fly’ continuous generation of alginate fibers using a microfluidic device,” Langmuir 23(17), 91049108 (2007).
5. H. Onoe, T. Okitsu, A. Itou, M. Kato-Negishi, R. Gojo, D. Kiriya, K. Sato, S. Miura, S. Iwanaga, and K. Kuribayashi-Shigetomi, “ Metre-long cell-laden microfibres exhibit tissue morphologies and functions,” Nat. Mater. 12(6), 584590 (2013).
6. A. Ilyas, M. Islam, W. Asghar, J. U. Menon, A. S. Wadajkar, K. T. Nguyen, and S. M. Iqbal, “ Salt-leaching synthesis of porous PLGA nanoparticles,” IEEE Trans. Nanotechnol. 12(6), 10821088 (2013).
7. A. Asthana, K. H. Lee, S.-J. Shin, J. Perumal, L. Butler, S.-H. Lee, and D.-P. Kim, “ Bromo-oxidation reaction in enzyme-entrapped alginate hollow microfibers,” Biomicrofluidics 5, 024117 (2011).
8. A. Asthana, K. H. Lee, K.-O. Kim, D.-M. Kim, and D.-P. Kim, “ Rapid and cost-effective fabrication of selectively permeable calcium-alginate microfluidic device using ‘modified’ embedded template method,” Biomicrofluidics 6, 012821 (2012).
9. Q. Liu, A. M. Rauth, and X. Y. Wu, “ Immobilization and bioactivity of glucose oxidase in hydrogel microspheres formulated by an emulsification-internal gelation-adsorption-polyelectrolyte coating method,” Int. J. Pharm. 339(1), 148156 (2007).
10. Y. A. Morch, I. Donati, B. L. Strand, and G. Skjåk-Bræk, “ Effect of Ca2+, Ba2+, and Sr2+ on alginate microbeads,” Biomacromolecules 7(5), 14711480 (2006).
11. A. Tamayol, M. Akbari, N. Annabi, A. Paul, A. Khademhosseini, and D. Juncker, “ Fiber-based tissue engineering: Progress, challenges, and opportunities,” Biotechnol. Adv. 31(5), 669687 (2013).
12. E. Kang, “ Novel PDMS cylindrical channels that generate coaxial flow, and application to fabrication of microfibers and particles,” Lab Chip 10(14), 18561861 (2010).
13. W. Jeong, J. Kim, S. Kim, S. Lee, G. Mensing, and D. J. Beebe, “ Hydrodynamic microfabrication via ‘on the fly’ photopolymerization of microscale fibers and tubes,” Lab Chip 4(6), 576580 (2004).
14. C. M. Hwang, A. Khademhosseini, Y. Park, K. Sun, and S.-H. Lee, “ Microfluidic chip-based fabrication of PLGA microfiber scaffolds for tissue engineering,” Langmuir 24(13), 68456851 (2008).
15. C. M. Hwang, Y. Park, J. Y. Park, K. Lee, K. Sun, A. Khademhosseini, and S. H. Lee, “ Controlled cellular orientation on PLGA microfibers with defined diameters,” Biomed. Microdevices 11(4), 739746 (2009).
16. A. Asthana, K.-O. Kim, J. Perumal, D.-M. Kim, and D.-P. Kim, “ Facile single step fabrication of microchannels with varying size,” Lab Chip 9(8), 11381142 (2009).
17. T. Takei, S. Sakai, H. Ijima, and K. Kawakami, “ Development of mammalian cell-enclosing calcium-alginate hydrogel fibers in a co-flowing stream,” Biotechnol. J. 1(9), 10141017 (2006).
18. S. Sakai, Y. Liu, E. J. Mah, and M. Taya, “ Horseradish peroxidase/catalase-mediated cell-laden alginate-based hydrogel tube production in two-phase coaxial flow of aqueous solutions for filament-like tissues fabrication,” Biofabrication 5(1), 015012 (2013).

Data & Media loading...


Article metrics loading...



Alginate is a natural polymer with inherent biocompatibility. A simple polydimethylsiloxane (PDMS) microfluidic device based self-assembled fabrication of alginate hollow microfibers is presented. The inner diameter as well as wall thickness of the microfibers were controlled effortlessly, by altering core and sheath flow rates in the microfluidic channels. The gelation/cross-linking occured while the solutions were ejected. The microfibers were generated spontaneously, extruding out of the outlet microchannel. It was observed that the outer diameter was independent of the flow rates, while the internal diameter and wall thickness of the hollow fibers were found to be functions of the core and sheath flow rates. At a constant sheath flow, with increasing core flow rates, the internal diameters increased and the wall thicknesses decreased. At a fixed core flow, when sheath flow rate increased, the internal diameters decreased and the wall thickness increased. The immobilization of enzymes in such hollow microfibers can be a potential application as microbioreactors.


Full text loading...


Access Key

  • FFree Content
  • OAOpen Access Content
  • SSubscribed Content
  • TFree Trial Content
752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd