Volume 96, Issue 10, 15 November 2004
Index of content:
- APPLIED BIOPHYSICS (PACS 87)
96(2004); http://dx.doi.org/10.1063/1.1803628View Description Hide Description
This paper presents a method for continuous magnetophoretic separation of red and white blood cells from whole blood based on their native magnetic properties. The microsystem separates the blood cells using a high gradient magnetic separation method without the use of additives such as magnetic tagging or inducing agents. A theoretical model of the magnetophoretic microseparator is derived and verified by comparison with finite element simulation. The microseparator is fabricated using microfabrication technology, enabling the integration of microscale magnetic flux concentrators in an aqueous microenvironment, providing strong magnetic forces, and fast separations. Experimental tests are performed using a permanent magnet to create an external magnetic flux of , and measuring the movement of red blood cells within the microchannel of the microseparator. The experimental results correlate well with the theoretical results.
96(2004); http://dx.doi.org/10.1063/1.1803629View Description Hide Description
Scanning probe microscopy was used to investigate the structural and electrical organization at the nanoscopic level of hydrated melanin thin films synthesized by oxidizing -3-(3,4-dihydroxyphenyl)-alanine (L-dopa) in dimethyl sulfoxide. Atomic force microscopy(AFM) provided the morphologies of the -dopa melanin films.Electrostatic force microscopy and conductive-AFM were used to spatially resolve the electrical properties of the material. Using a simple parallel plate capacitor model a method to measure the charge distribution on the sample was developed. The correlations between topography, electric charge, and current images of the sample demonstrated that the hydration process produces a restructuring of melanin observed not only through topographic variations, but also through the creation of areas with different electrical properties.
96(2004); http://dx.doi.org/10.1063/1.1803925View Description Hide Description
A method is presented to describe the behavior of an oscillating bubble in a fluid near a second elastic (biological) fluid. The elasticity of the second fluid is modeled through a pressure term at the interface between the two fluids. The Laplace equation is assumed to be valid in each of the fluids, and a difference in the respective densities is allowed. A relationship between the two velocity potentials just above and below the fluid-fluidinterface can be found. The boundary integral method is then used to solve for the unknown normal velocities at both the bubble interface and fluid-fluidinterface. These said normal velocities are subsequently utilized to update the position of the interface(s) for the next time step. For bubbles oscillating near a second nonelastic fluid, the bubbles can develop a jet towards or away from the fluid-fluidinterface (depending on the distance of the bubble from the fluid-fluidinterface and the density ratios of the two fluids). This behavior can be greatly modified when the second fluid possesses some elastic properties. The elasticity causes a small perturbation to travel along the surface of the bubble. A complex interaction between this growing perturbation and the bubble in its collapse phase can lead to the bubble assuming a “mushroom” shape and/or even breakup into two smaller bubbles. These phenomena have not been observed when the elasticity is absent in the second fluid. Excellent agreement with experimental data was obtained for a wide range of parameters.