Experimental setup scheme.
(Color online (a) TIRFM image of streptavidin functinonalized (CdSe)ZnS QDs immobilized on a glass coverslip. (b) Intensity time traces of selected individual QDs. (c) Corresponding normalized intensity time correlation function of single QDs’ intensity time trace presented in (b).
Normalized intensity time correlation functions for various laser excitation powers as calculated from 2000 frame image time series with /frame integration time for blinking streptavidin functionalized (CdSe)ZnS QDs on a glass substrate. (∎), (∎), and (◻) excitation laser powers. Intensity correlation functions are normalized to 1 for comparison.
Plot of as a function of laser power. Each value is an average from 15 measurements. The error bars are standard deviations.
Normalized intensity correlation functions calculated from 1000 frame simulated image series with an image time step of . Each image consists of 300 point emitters. The “” time probability distribution exponent is 1.5 for all simulated image series. The “on” time distribution exponents are 1.5 (∎), 1.7 (∎), and 1.9 (◻) respectively.
Summary of the mean fitting exponent for the normalized intensity correlation functions of simulated image time series with different values and for different number of dots ranging from 800 to 25. is set to 1.5. The number of blinking point emitters is included in brackets. Each value is an average from 20 simulations. Uncertainties are standard deviations.
Summary of the mean fitting exponent for the normalized intensity correlation functions of simulated image time series for an ensemble containing 150 point emitters with different values and for different number of frames ranging from 100 to 10 000. is set to 1.5. Each value is an average from 20 simulations. Uncertainties are standard deviations.
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