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In situ observation of DNA hybridization and denaturation by surface infrared spectroscopy
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10.1063/1.2188128
/content/aip/journal/jap/99/9/10.1063/1.2188128
http://aip.metastore.ingenta.com/content/aip/journal/jap/99/9/10.1063/1.2188128
View: Figures

Figures

Image of FIG. 1.
FIG. 1.

Biocell for MIR-IRAS measurements.

Image of FIG. 2.
FIG. 2.

Time evolution of the cell solution temperature with different voltages applied sequentially to the electrodes on the backside of the Si prism.

Image of FIG. 3.
FIG. 3.

IRAS spectra measured for the noncomplementary pair of oligonucleotides, in a solution of NaCl in . (a) A mixed solution of and , and solutions of (b) and (c) .

Image of FIG. 4.
FIG. 4.

IRAS spectra measured for the complementary pair of oligonucleotides, in a solution of NaCl in . (a) a mixed solution of and and solutions of (b) and (c) .

Image of FIG. 5.
FIG. 5.

Denature curve and the temperature dependence of the ratio of the IR absorbance at to that at for the complementary DNA pair of .

Image of FIG. 6.
FIG. 6.

Series of IRAS spectra of the DNA pair of measured in a solution of NaCl in for different solution temperatures.

Image of FIG. 7.
FIG. 7.

Denature curve and the temperature dependence of IR absorbance ratio for the complementary pair of . has a four-based mismatch to .

Image of FIG. 8.
FIG. 8.

Series of IRAS spectra of a solution of the DNA pair of measured in a solution of NaCl in for different solution temperatures.

Image of FIG. 9.
FIG. 9.

Base pairs of DNA. (a) Adenine-thymine and (b) guanine-cytosine.

Image of FIG. 10.
FIG. 10.

Calculated oscillator strengths for the base pairs and the individual bases.

Image of FIG. 11.
FIG. 11.

Calculated and experimental IRAS spectra of the individual bases.

Image of FIG. 12.
FIG. 12.

Calculated IRAS spectra of (a) the A-T pair, (b) the G-C pair, and (c) the sum of the two pairs. Thin curves indicate the computed spectra of the separated base pairs. A dotted curve indicates an IRAS spectrum measured for the complementary pair of .

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/content/aip/journal/jap/99/9/10.1063/1.2188128
2006-05-15
2014-04-25
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: In situ observation of DNA hybridization and denaturation by surface infrared spectroscopy
http://aip.metastore.ingenta.com/content/aip/journal/jap/99/9/10.1063/1.2188128
10.1063/1.2188128
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