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Observation of electrostatically released DNA from gold electrodes with controlled threshold voltages
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19.About double stranded oligo DNA: We suppose that the DNA loaded on the electrode are double stranded though 12mer DNA is not long enough to hybridize tightly. Because, same phenomenon was observed in experiments in which a ratio of labeled DNA and non-labeled complementary DNA is varied to modify condition of hybridization.
19.About PCR product: PCR product used in the experiment was labeled by using a primer DNA bound with a fluorescent dye. And it still remains to be examined whether residual primer DNA was removed sufficiently before the experiment or not. But a remarkable decrease in the fluorescent intensity was confirmed.
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