Structure of 8-anilino-1-naphtalenesulfonate, ammonium salt (ANS).
Tryptophan emission of BSA in the presence of, respectively [(a)–(g)] 0, 5, 10, 15, 20, 25, and ANS . The peak at corresponds to ANS.
Stern Volmer plot for quenching of tryptophan emission of BSA by ANS.
Absorption (-----) and emission (—) spectra of ANS in an aqueous solution containing BSA at .
Fluorescence anisotropy decay of ANS ( and ) in (a) BSA at (initial part) and (b) methanol at .
Fluorescence anisotropy decay of ANS ( and ) in BSA at . The points denote the actual values of anisotropy and the solid lines denote the best fit to the experimental data. The inset shows the best fit to the anisotropy decays at [(a)–(d)] 278, 293, 303, and .
Fluorescence decays of ANS in BSA in water at at the emission wavelengths (i) , (ii) , and (iii) .
Time resolved emission spectra of ANS in BSA in water at (∎), (엯), (▴), and (▿) at .
Decay of solvent response function of ANS in BSA in water at (▿), (▴), (엯), and (∎). The points denote the actual values of , and the solid lines denote the best fit to the experimental data.
Plot of against .
Parameters of fluorescence anisotropy decay (monitored at ) of ANS in BSA at different temperatures.
Parameters obtained from decay of for ANS in BSA at different temperatures.
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