1887
banner image
No data available.
Please log in to see this content.
You have no subscription access to this content.
No metrics data to plot.
The attempt to load metrics for this article has failed.
The attempt to plot a graph for these metrics has failed.
Temperature dependence of solvation dynamics and anisotropy decay in a protein: ANS in bovine serum albumin
Rent:
Rent this article for
USD
10.1063/1.2178782
/content/aip/journal/jcp/124/12/10.1063/1.2178782
http://aip.metastore.ingenta.com/content/aip/journal/jcp/124/12/10.1063/1.2178782

Figures

Image of Scheme 1.
Scheme 1.

Structure of 8-anilino-1-naphtalenesulfonate, ammonium salt (ANS).

Image of FIG. 1.
FIG. 1.

Tryptophan emission of BSA in the presence of, respectively [(a)–(g)] 0, 5, 10, 15, 20, 25, and ANS . The peak at corresponds to ANS.

Image of FIG. 2.
FIG. 2.

Stern Volmer plot for quenching of tryptophan emission of BSA by ANS.

Image of FIG. 3.
FIG. 3.

Absorption (-----) and emission (—) spectra of ANS in an aqueous solution containing BSA at .

Image of FIG. 4.
FIG. 4.

Fluorescence anisotropy decay of ANS ( and ) in (a) BSA at (initial part) and (b) methanol at .

Image of FIG. 5.
FIG. 5.

Fluorescence anisotropy decay of ANS ( and ) in BSA at . The points denote the actual values of anisotropy and the solid lines denote the best fit to the experimental data. The inset shows the best fit to the anisotropy decays at [(a)–(d)] 278, 293, 303, and .

Image of FIG. 6.
FIG. 6.

Fluorescence decays of ANS in BSA in water at at the emission wavelengths (i) , (ii) , and (iii) .

Image of FIG. 7.
FIG. 7.

Time resolved emission spectra of ANS in BSA in water at (∎), (엯), (▴), and (▿) at .

Image of FIG. 8.
FIG. 8.

Decay of solvent response function of ANS in BSA in water at (▿), (▴), (엯), and (∎). The points denote the actual values of , and the solid lines denote the best fit to the experimental data.

Image of FIG. 9.
FIG. 9.

Plot of against .

Tables

Generic image for table
Table I.

Parameters of fluorescence anisotropy decay (monitored at ) of ANS in BSA at different temperatures.

Generic image for table
Table II.

Parameters obtained from decay of for ANS in BSA at different temperatures.

Loading

Article metrics loading...

/content/aip/journal/jcp/124/12/10.1063/1.2178782
2006-03-28
2014-04-16
Loading

Full text loading...

This is a required field
Please enter a valid email address
752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Temperature dependence of solvation dynamics and anisotropy decay in a protein: ANS in bovine serum albumin
http://aip.metastore.ingenta.com/content/aip/journal/jcp/124/12/10.1063/1.2178782
10.1063/1.2178782
SEARCH_EXPAND_ITEM