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Dissociation of heme from gaseous myoglobin ions studied by infrared multiphoton dissociation spectroscopy and Fourier-transform ion cyclotron resonance mass spectrometry
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10.1063/1.2221696
/content/aip/journal/jcp/125/13/10.1063/1.2221696
http://aip.metastore.ingenta.com/content/aip/journal/jcp/125/13/10.1063/1.2221696
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Figures

Image of FIG. 1.
FIG. 1.

FTICR mass spectra of protonated holomyoglobin ions produced from ESI of protein solutions (a) without any pretreatment and (b) pretreated with L-ascorbic acid for . The peaks denoted by “∗” in (b) correspond to protonated apomyoglobin ions with .

Image of FIG. 2.
FIG. 2.

Time sequences of pulse events in (a) SORI-CID and (b) IRMPD experiments.

Image of FIG. 3.
FIG. 3.

SORI-CID mass spectra of at different levels of rf attenuation (in dB). The fragment ions and result from charged heme loss and neutral heme loss, respectively.

Image of FIG. 4.
FIG. 4.

Fractions of charged (◻) vs neutral (엯) heme loss of , and their ratios (∎), as a function of SORI excitation energy. The dissociation fraction is defined as , where and are the intensities of precursor ions and fragment ions, respectively.

Image of FIG. 5.
FIG. 5.

IRMPD spectra of with (×), 10 (∎), and 11 (엯). The peak intensities represent dissociation fractions as defined in Fig. 4.

Image of FIG. 6.
FIG. 6.

IRMPD of pure ferric . (a) Time trace of charged heme loss at each of five laser intensities for the ion only. (b) Plot of the natural logarithm of the first-order unimolecular dissociation rate constant, , vs the natural logarithm of the laser intensity (in units of ). The fitted slopes are , , and at , 11, and 12, respectively. The frequency of the OPO laser excitation was fixed at .

Image of FIG. 7.
FIG. 7.

Plot of the natural logarithm of the dissociation rate constants of charged vs neutral heme loss of (∎,◻) and (●,엯) as a function of the natural logarithm of the laser intensity (in units of ). The laser excitation frequency was fixed at . The fitted slope of charged vs neutral heme loss is vs , respectively.

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/content/aip/journal/jcp/125/13/10.1063/1.2221696
2006-10-03
2014-04-17
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Dissociation of heme from gaseous myoglobin ions studied by infrared multiphoton dissociation spectroscopy and Fourier-transform ion cyclotron resonance mass spectrometry
http://aip.metastore.ingenta.com/content/aip/journal/jcp/125/13/10.1063/1.2221696
10.1063/1.2221696
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