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Effect of cholesterol on diffusion in surfactant bilayers
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Image of FIG. 1.
FIG. 1.

Phase diagram of and . and denote the micellar and the lamellar phase, respectively. is the hexagonal phase, and and the inverse micellar and the solid phase. is the cubic phase. is a sponge phase. Unnamed areas are bicontinuous. The component ratios that were measured are marked with gray dots. The image was redrawn from Mitchell et al. (Ref. 27).

Image of FIG. 2.
FIG. 2.

Structures of (a) R18 and (b) cholesterol.

Image of FIG. 3.
FIG. 3.

Sketch of the orientation model: In the lamellar phase, molecular diffusion is thought to be restricted to two-dimensional planes intersecting the focus intensity distribution at arbitrary angles and vertical intercepts. Integrating the contributions from different orientations results in an effective angle .

Image of FIG. 4.
FIG. 4.

Confocal microscope setup for FCS and schematic of the observation volume with half-axes and (enlarged).

Image of FIG. 5.
FIG. 5.

FCS data for the micellar phase. The diffusion time in this example is .

Image of FIG. 6.
FIG. 6.

Example for the FCS data from the surfactant/cholesterol/water emulsion. The experimental data (crosses) are compared to best fits using the standard (single-component) model (dashed-dotted line) and to the orientation model (full line). The cholesterol to surfactant ratio was 0.172. The diffusion times are (standard model) and (orientation model).

Image of FIG. 7.
FIG. 7.

Result for the diffusion coefficient of R18 in the lamellar phase with surfactant. The molar ratio of cholesterol to surfactant increases to the right. A reduction in molecular mobility is apparent at a ratio of 0.08. Error bars denote the standard deviation. Guidelines for the eyes are drawn as dashed lines.

Image of FIG. 8.
FIG. 8.

Diffusion coefficients for the surfactant emulsion as a function of the cholesterol content.

Image of FIG. 9.
FIG. 9.

Counts/molecule value (cpm) as a function of cholesterol content from the same sample on two different days. The brightness is reduced by increasing cholesterol content.


Generic image for table
Table I.

The table subsumes the amount of substances used in the microemulsions. The ratio value gives the molar ratio of cholesterol vs . The lipid frac. value denotes the fraction of and cholesterol.

Generic image for table
Table II.

Overview of diffusion coefficients in /water/cholesterol emulsions determined from the orientation model.


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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Effect of cholesterol on diffusion in surfactant bilayers