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Diffusion and segmental dynamics of rodlike molecules by fluorescence correlation spectroscopy
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10.1063/1.2753160
/content/aip/journal/jcp/127/5/10.1063/1.2753160
http://aip.metastore.ingenta.com/content/aip/journal/jcp/127/5/10.1063/1.2753160
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Figures

Image of FIG. 1.
FIG. 1.

(Color online) Mean square displacement (MSD) of a semiflexible polymer according to Eq. (27). The top line is the total MSD, the bottom line the center-of-mass MSD, and the curve reaching a plateau the intramolecular MSD. The dotted line is the intramolecular part of the analytical expression (39) (last term). The dashed line is the MSD of the rotational motion of a rod [Eq. (53)].

Image of FIG. 2.
FIG. 2.

FCS correlation functions for semiflexible polymers of various lengths: , , , , and . The solid lines are determined including all modes (polymer length increases from left to right), whereas for the dotted lines the center-of-mass motion is omitted (length increases from top to bottom).

Image of FIG. 3.
FIG. 3.

FCS correlation functions for semiflexible polymers of various lengths: , , , and (from left to right). The solid lines are determined including all internal modes, whereas for the dotted lines the first mode (rotational motion) is suppressed. The inset shows the same comparison without center-of-mass motion (length increases from top to bottom). The two shortest lengths yield and are hence indistinguishable in the inset.

Image of FIG. 4.
FIG. 4.

(Color online) FCS correlation function of actin filaments. The scattered curve is taken from the experimental results presented in Ref. 15 and the smooth line has been calculated using Eqs. (46) and (47). The filament length is , , and the thickness .

Image of FIG. 5.
FIG. 5.

Comparison of the exact theoretical FCS correlation function of actin filaments (solid line) with the analytical approximation (49) (dotted line).

Image of FIG. 6.
FIG. 6.

(Color online) Average mean square displacement [Eq. (27)] of actin filaments. The top solid line is the theoretical result of the total mean square displacement and the bottom line is the mean square displacement in the center-of-mass reference frame. The symbols are experimental values taken from Ref. 15. Note, the time scale of the experiment is devided by 1.7.

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/content/aip/journal/jcp/127/5/10.1063/1.2753160
2007-08-06
2014-04-17
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Diffusion and segmental dynamics of rodlike molecules by fluorescence correlation spectroscopy
http://aip.metastore.ingenta.com/content/aip/journal/jcp/127/5/10.1063/1.2753160
10.1063/1.2753160
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