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Polymer induced condensation of DNA supercoils
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10.1063/1.2998521
/content/aip/journal/jcp/129/18/10.1063/1.2998521
http://aip.metastore.ingenta.com/content/aip/journal/jcp/129/18/10.1063/1.2998521
View: Figures

Figures

Image of FIG. 1.
FIG. 1.

Condensation assay. Mixtures with a fixed concentration of DNA and an increasing concentrations of PEO are centrifuged for at . The supernatant is analyzed with agarose gel electrophoresis. The figure shows typical gels for . Lane 1: without PEO 20K; lanes 2–12: PEO 20K concentration increasing from . (a) Supercoiled pUC 18, the faint band is a small amount of nicked DNA. (b) EcoRI linearized pUC18.

Image of FIG. 2.
FIG. 2.

Condensation assay. Intensity of the DNA bands as a function of wt % PEO 20K, again at . Squares: EcoRI linearized pUC18. Circles: supercoiled pUC18.

Image of FIG. 3.
FIG. 3.

Condensation treshold (PEO 20K wt % at which the intensity of the DNA bands in the condensation assay start decreasing) as a function of [NaCl]. Squares: EcoRI linearized pUC18. Circles: supercoiled pUC18.

Image of FIG. 4.
FIG. 4.

Monte Carlo simulations of small DNA circles in nematic fields, at two different superhelical densities: (squares) and (triangles). (a) Superhelical diameter as a function of the strength of the nematic potential. (b) Writhe per added link as a function of the strength of the nematic potential.

Image of FIG. 5.
FIG. 5.

Snapshot of typical configurations of the DNA circles during the Monte Carlo simulations in the absence (top) and the presence (bottom) of a strong nematic field.

Image of FIG. 6.
FIG. 6.

Monte Carlo simulations of small DNA circles with an added depletion attraction between the beads representing the DNA, at two different superhelical densities: (squares) and (triangles). (a) Superhelical diameter as a function of the strength of the depletion potential between the beads. (b) Writhe per added link as a function of .

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/content/aip/journal/jcp/129/18/10.1063/1.2998521
2008-11-10
2014-04-20
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Polymer induced condensation of DNA supercoils
http://aip.metastore.ingenta.com/content/aip/journal/jcp/129/18/10.1063/1.2998521
10.1063/1.2998521
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