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Measuring distances within unfolded biopolymers using fluorescence resonance energy transfer: The effect of polymer chain dynamics on the observed fluorescence resonance energy transfer efficiency
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10.1063/1.3212602
/content/aip/journal/jcp/131/8/10.1063/1.3212602
http://aip.metastore.ingenta.com/content/aip/journal/jcp/131/8/10.1063/1.3212602
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Figures

Image of FIG. 1.
FIG. 1.

The observed FRET efficiency increases with the intrinsic lifetime of the donor, , which is the lifetime of the donor in the absence of the acceptor. As shown, this effect is more pronounced for shorter polymer chains than for longer chains . Note that the Förster radius employed here was chosen such that in the static limit , the limit in which Eq. (3) is an accurate approximation, the FRET efficiency of both cases is the same.

Image of FIG. 2.
FIG. 2.

Polymers differing in length ( and ) exhibit (nearly) the same dependence on chain dynamics when the observed FRET efficiency is plotted as a function the dimensionless ratio of donor lifetime and the polymer reconfiguration time (data as in Fig. 1). The solid line is a guide to the eye.

Image of FIG. 3.
FIG. 3.

The FRET efficiency calculated from Eqs. (6)–(9) (circles) deviates from the prediction of the commonly employed Eq. (3) (squares) even when the donor lifetime is considerably shorter than time scale associated with polymer fluctuations (here and . Of note is that these curves are virtually independent of the chain length). Here, horizontal lines correspond to and .

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/content/aip/journal/jcp/131/8/10.1063/1.3212602
2009-08-28
2014-04-19
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Measuring distances within unfolded biopolymers using fluorescence resonance energy transfer: The effect of polymer chain dynamics on the observed fluorescence resonance energy transfer efficiency
http://aip.metastore.ingenta.com/content/aip/journal/jcp/131/8/10.1063/1.3212602
10.1063/1.3212602
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