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Statistical analysis of time resolved single molecule fluorescence data without time binning
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View: Figures


Image of FIG. 1.
FIG. 1.

Sequence of single photon counts measured with two detectors being sensitive to two orthogonal polarizations.

Image of FIG. 2.
FIG. 2.

Comparison of the rotational correlation function from simulated time binned data with the correlation analysis without preceding time binning of the data . For each an individual had been chosen fulfilling .

Image of FIG. 3.
FIG. 3.

Excitation laser pulse sequence for TCSPC. The delay times for individually detected photons (solid bar) are recorded.

Image of FIG. 4.
FIG. 4.

Simple two state model of time dependent fluorescence decay rates. For a given state an exponential delay time distribution is assumed. Exchange is slow compared to fluorescence decay .

Image of FIG. 5.
FIG. 5.

PDFs calculated according to Eqs. (7) and (8) with and , see text. While is independent of , the long time limit broadens with increasing ratio or .

Image of FIG. 6.
FIG. 6.

Simulated data analyzed with the correlation function . A simple two state model had been assumed with and , see text. From a Levenberg–Marquardt fit to the data (solid line) the input parameters could be recovered, demonstrating the suitability of the correlation function .


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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Statistical analysis of time resolved single molecule fluorescence data without time binning