Structures for nicotinamide adenine dinucleotide (NAD+), left, and azido-nicotinamide adenine dinucleotide (azido-NAD+), right.
Spectral slices of the dispersed pump-probe decay for delay times T = 500 fs (red), 1 ps (blue), and 2 ps (black).
Decay of the excited-state absorption contribution to the dispersed pump-probe spectrum as a function of the pump-probe delay. The transient is fit to a single exponential decay with a time constant of 1.0 ps.
2D-IR spectra of azido-NAD+ for population times T = 0 fs, 500 fs, and 1.1 ps. The open circles mark the center lines and the solid red lines are the linear fits to the centerlines that we use to determine the CLS.
CLS vs waiting time (open circles) along with the exponential fit to the CLS decay. The inset compares the measured (red) and calculated (black) antisymmetric stretch absorption spectrum of the azido-NAD+ chromophore. The structure of Azido-NAD+ is also shown (ADPR: adenine pyrophosphate).
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