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Making novel bio-interfaces through bacterial protein recrystallization on biocompatible polylactide derivative films
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10.1063/1.4811778
/content/aip/journal/jcp/139/12/10.1063/1.4811778
http://aip.metastore.ingenta.com/content/aip/journal/jcp/139/12/10.1063/1.4811778

Figures

Image of FIG. 1.
FIG. 1.

Thermograms obtained by DSC showing the glass transitions (T) of polymers and crystallization (T) and melting peak (T) in PLLA.

Image of FIG. 2.
FIG. 2.

Tensile stress-strain curves of PLLA, PLGA, PDLLA, and PLCL at T = 23 °C. The inset (see the lupe) in a zoom of the main figure to show in detail the stress-strain curves for PLLA, PLGA, and PDLLA.

Image of FIG. 3.
FIG. 3.

Frequency (ΔF) and dissipation (ΔD) response of SbpA adsorption on PLLA (black), PDLLA (red), PLGA (green), and PLCL (blue) at 23 °C. The numbered windows (1–4) refer to the different effects observed in dissipation during S-layer formation.

Image of FIG. 4.
FIG. 4.

Dissipation vs. frequency curves for S-layer recrystallization on PLLA (black), PDLLA (red), PLGA (green), and PLCL (blue). The numbers indicate the three different processes observed in S-layer formation. The maximum in dissipation reaches within 30 s (t1) while the minimum occurs 1 h (t2) after SbpA injection.

Image of FIG. 5.
FIG. 5.

AFM height images of SbpA recrystallized on (a) PLLA, (b) PDLLA, (c) PLGA, (d) PLCL after 12 h of protein incubation and at room temperature. The black shapes delimit the protein domains of size, 0.011 m for PLLA, 0.034 m for PDLLA, 0.036 m for PLGA, and 0.09 m for PLCL and also show the differences in protein orientation. The bottom right insets are the FFT images which reveal the periodicity of the S-layer. The lattice parameters calculated from FFT demonstrate S-layer has square lattice symmetry (p4) in those biopolymers (see Table IV for details).

Tables

Generic image for table
Table I.

Characteristics of the biopolymers used as substrates.

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Table II.

Mechanical properties of PLLA, PDLLA, PLGA, and PLCL.

Generic image for table
Table III.

Domain size and roughness of S-layers obtained by AFM. Mass adsorption, dissipation, and initial adsorption rate calculated from QCM-D curves.

Generic image for table
Table IV.

Roughness (R) and lattice parameters (a, b, and γ) of recrystallized SbpA on PLLA, PDLLA, PLGA, and PLCL calculated from AFM height images.

Generic image for table
Table V.

PLLA, PDLLA, PLGA, and PLCL films properties; glass transition temperature (T), contact angle (ø), and roughness (R). The roughness was calculated from AFM height images.

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/content/aip/journal/jcp/139/12/10.1063/1.4811778
2013-07-01
2014-04-24
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Making novel bio-interfaces through bacterial protein recrystallization on biocompatible polylactide derivative films
http://aip.metastore.ingenta.com/content/aip/journal/jcp/139/12/10.1063/1.4811778
10.1063/1.4811778
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