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Rapid Scanning Microspectrofluorimeter
1.L. N. M. Duysens and J. Amesz, Biochim et Biophys. Acta 24, 19 (1957).
2.J. M. Olson, Brookhaven Symposia in Biol. 11, 316 (1958).
3.B. Chance and V. Legallais, Rev. Sci. Instr. 30, 732 (1959).
4.R. A. Olson, C. L. Greenblatt, and E. K. Engel, presented at the Biophysical Society Meeting, 1958, Pittsburgh, Pennsylvania (to be published).
5.J. Sjöstrand, Acta Physiol. Scand. 12, 42 (1946).
6.A double linear spiral slit was used in scanning spectrophotometry, in unpublished work by F. S. Brackett (1940).
7.The conventional employment of dark field optics to exclude the direct exciting light from the optical axis was avoided in order to retain the advantage of objectives with high numerical aperture for resolution and light gathering power.
8.A Zeiss hand spectroscope, model D, or equivalent, can be substituted here, if more convenient.
9.The spectral changes induced in chloroplasts by the intense exciting light used here are photooxidative and can be controlled by denial of oxygen. In the use of such high intensity for other systems with low fluorescent yields, photochemical sensitivity and its control must likewise be considered.
10.Computed from that of two identical condensers focused and immersed face to face.
11.R. Emerson and W. Arnold, J. Gen. Physiol. 6, 191 (1932).
12.R. A. Olson and E. K. Engel, Brookhaven Symposia in Biol. 11, 303 (1958).
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