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Fluorescence polarimeter for flow cytometry
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9.The total emitted fluorescent intensity is proportional to For that reason the anisotropy may be preferred to the commonly used as a measure of the degree of polarization.
10.G. Weber, Biochem. 51, 145 (1952). This is a rearrangement of his Eq. (34). Our measurements accurately show this behavior.
11.Model FRD‐33 Continental Optical Corp., 15 Power Dr., Hauppauge, NY 11787. The two pieces are optically contacted rather than cemented to minimize beam motion during rotations.
12.Spectra Physics model 164.
13.Model SBTA‐8‐45 Karl Lambrecht, 4318 West Lincoln Avenue, Chicago, IL 60618.
14.Model 9798B EMI Gencom, 80 Express Street, Plainview, NY 11803.
15.Pactra GI white glass frosting, Pactra Industries, Inc., Los Angeles, CA 90028.
16.Northern Scientific Company model NS 621 modified to be compatible with the DR11‐K interface.
17.As mentioned below, further processing involves arrays with reduced resolution, and since the polarization is calculated immediately, a 1024 by 1024 array is never formed.
18.Digital Equipment Corporation, Maynard, MA.
19.Listings of these programs are available on request.
20.It is possible that photocathode variations coupled with variations in the path of the cells through the laser beam will result in correlations between the signals in the two detectors that are more complicated than the simple proportional relationship emphasized here, for example, one decreasing while the other increases. Adequate diffusers remove these.
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