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A nondestructive method for determining the spring constant of cantilevers for scanning force microscopy
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16.The micropipettes were formed by pulling 1 mm glass tubules over a small bunsen burner flame. Using this method, micropipettes with micrometer-sized diameters could be fabricated. We found that pipettes that necked down to the smallest diameter over a short distance worked well since they resisted bending.
17.Variations in the elastic modulus or density are also a possibility. Since the cantilevers are made using chemical-vapor deposition, the exact stoichiometry is not known, The range can be as high as [A. L. Weisenhorn, P. Maivald, H. J. Butt, and P. K. Hansma, Phys. Rev. B 45, 11 226 (1992)]. However, for the measured values of spring constant and resonant frequency to remain consistent with Eqs. (1) and (3), the elastic modulus would have to vary by a factor of 6 and the denstiy by 50%.
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