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Optical scanning instrument for ultrafast pump-probe spectroscopy of biomolecules at cryogenic temperatures
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View: Figures


Image of FIG. 1.
FIG. 1.

The conventional pump-probe experimental setup where the sample is rotating in the focal plane of the focusing lens. “A” is a polarization analyzer.

Image of FIG. 2.
FIG. 2.

The lens spinning geometry. The lens spins around an off-center axis of rotation (radius ). Two lens center positions are shown [(a) and (b)]. The corresponding focal point motion follows the spinning lens center.

Image of FIG. 3.
FIG. 3.

The modified pump-probe experimental setup. The sample is stationary and is positioned in the spinning lens focal plane. Two cascaded lock-ins are used for background suppression. The pulse picker is used to lower the laser repetition rate as needed. “A” is a polarization analyzer.

Image of FIG. 4.
FIG. 4.

MbNO kinetics at . Glycerol 75% (blue) and water (red) solvents are used for the sample preparation. The inset shows the rate distribution obtained after the MEM analysis for the two samples that were studied using the sample scanning method (squares) and the lens spinning technique (circles).

Image of FIG. 5.
FIG. 5.

Deoxy Mb FCS. The three data sets show comparisons between different scans.

Image of FIG. 6.
FIG. 6.

Dynamics of the NO molecule recombination following photolysis of MbNO as a function of temperature along with the dependence of the corresponding maximum entropy distribution. The MEM amplitudes are measured linearly on the right axis. Vertical and horizontal arrows indicate the kinetics tendency and the “slow” phase rate distribution tendency, respectively, as the temperature is lowered. The MEM amplitudes for are shifted vertically upwards for clarity because they display a decreased recombination rate at higher temperature due to protein relaxation effects.

Image of FIG. 7.
FIG. 7.

Deoxy Mb. FCS measurements as a function of temperature.


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Scitation: Optical scanning instrument for ultrafast pump-probe spectroscopy of biomolecules at cryogenic temperatures