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High-energy collision induced dissociation fragmentation pathways of peptides, probed using a multiturn tandem time-of-flight mass spectrometer “MULTUM-TOF/TOF”
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10.1063/1.2751403
/content/aip/journal/rsi/78/7/10.1063/1.2751403
http://aip.metastore.ingenta.com/content/aip/journal/rsi/78/7/10.1063/1.2751403

Figures

Image of FIG. 1.
FIG. 1.

Nomenclature of the fragments produced from linear peptides as described by Roepstorff and Fohlman (Ref. 1).

Image of FIG. 2.
FIG. 2.

The possibilities for the production of different and ions arising from branched and unbranched amino-acid side chains.

Image of FIG. 3.
FIG. 3.

Schematic drawing of the tandem TOF mass spectrometer MULTUM-TOF/TOF.

Image of FIG. 4.
FIG. 4.

(Color online) Photograph of the tandem TOF mass spectrometer MULTUM-TOF/TOF. MALDI ion source is shown on the left, MULTUM mass spectrometer (MS1) on the center, and the quadratic-field ion mirror (MS2) on the right.

Image of FIG. 5.
FIG. 5.

Schematic drawing of the MALDI ion source of the MULTUM-TOF/TOF.

Image of FIG. 6.
FIG. 6.

(Color online) Ion trajectories of the MULTUM II geometry multiturn TOF mass spectrometer simulated by the computational program TRIO-DRAW (Ref. 30): (a) top view, (b) direction, and (c) direction.

Image of FIG. 7.
FIG. 7.

(Color online) Photograph of the multiturn TOF mass analyzer (MS1). The circle in the center defines the field-free region between the electric sectors.

Image of FIG. 8.
FIG. 8.

(Color online) (a) Photograph and (b) isometric drawing of the quadratic-field ion mirror (MS2). The ion mirror consists of 33 elements placed at unequal distances in order to define the field with the smaller number of different applied voltages.

Image of FIG. 9.
FIG. 9.

Block diagram of the timing control system.

Image of FIG. 10.
FIG. 10.

MALDI-TOF spectra of angiotensin I. (a) Whole isotope distribution of angiotensin I after . (b) Monoisotopic ion has been isolated and transmitted.

Image of FIG. 11.
FIG. 11.

High-energy CID spectrum of angiotensin I (DRVYIHPFHL) (a) from all isotopes of angiotensin I (after linear 0 cycle) and (b) from monoisotopic peak (after ). Ions characteristic of high-energy collisions are observed on both spectra.

Image of FIG. 12.
FIG. 12.

High-energy CID spectrum of substance P (RPKPQQFFGLM-) from monoisotopic peak after circulating in the MULTUM (about ).

Image of FIG. 13.
FIG. 13.

High-energy CID spectrum of bradykinin (RPPGFSPFR) from monoisotopic peak after circulating in the MULTUM (about ).

Image of FIG. 14.
FIG. 14.

Reaction pathway for the production of , , and fragments.

Image of FIG. 15.
FIG. 15.

Fragmentation cascade at high-energy collisions produces series from or the less abundant series.

Tables

Generic image for table
Table I.

The calculated distances of electrodes and applied voltages.

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/content/aip/journal/rsi/78/7/10.1063/1.2751403
2007-07-02
2014-04-17
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: High-energy collision induced dissociation fragmentation pathways of peptides, probed using a multiturn tandem time-of-flight mass spectrometer “MULTUM-TOF/TOF”
http://aip.metastore.ingenta.com/content/aip/journal/rsi/78/7/10.1063/1.2751403
10.1063/1.2751403
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