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Time-resolved scanning system for double reflectance and transmittance fluorescence imaging of diffusive media
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10.1063/1.2828054
/content/aip/journal/rsi/79/1/10.1063/1.2828054
http://aip.metastore.ingenta.com/content/aip/journal/rsi/79/1/10.1063/1.2828054
View: Figures

Figures

Image of FIG. 1.
FIG. 1.

System setup schematic.

Image of FIG. 2.
FIG. 2.

Example of a recorded curve: (a) fitting curve for optical parameters recovery. (b) Integration ranges for time gated fluorescence signal evaluation.

Image of FIG. 3.
FIG. 3.

Linearity of the estimated optical parameters of the bulk solution. (a) Estimated against nominal ; (b) estimated against nominal .

Image of FIG. 4.
FIG. 4.

Cross correlation of the estimated optical parameters (a) estimated against nominal ; (b) estimated against .

Image of FIG. 5.
FIG. 5.

Fluorescence signal linearity against dye concentration in the bulk solution. The continuous lines represent linear fits of the data.

Image of FIG. 6.
FIG. 6.

Fluorescence images in transmittance geometry: (a) inclusion concentration , depth ; (b) inclusion concentration , depth ; (c) inclusion concentration , depth ; (d) inclusion concentration , depth ; (e) inclusion concentration , depth ; (f) inclusion concentration , depth .

Image of FIG. 7.
FIG. 7.

Fluorescence images in reflectance geometry: (a) inclusion concentration , depth ; (b) inclusion concentration , depth ; (c) inclusion concentration , depth ; (d) inclusion concentration , depth ; (e) inclusion concentration , depth ; (f) inclusion concentration , depth .

Image of FIG. 8.
FIG. 8.

Late gate fluorescence signal against inclusion concentration for the images: (a) side 1 reflectance; (b) transmittance injecting from side 0 and collecting from side 1.

Image of FIG. 9.
FIG. 9.

Image contrasts: (a) against fluorophore concentration inside the inclusion, for different inclusion positions; (b) against inclusion position for different concentrations of the dye. For both figures, signals recorded on PMT1 in transmittance geometry.

Image of FIG. 10.
FIG. 10.

Comparison between gated images and a cw one: (a) early gate image; (b) cw image; (c) late gate image.

Image of FIG. 11.
FIG. 11.

Fluorescence signal dependence on inclusion position for different inclusion concentrations: (a) transmittance geometry; (b) reflectance geometry.

Image of FIG. 12.
FIG. 12.

Fluorescence lifetime estimation by means of a linear fit of the curve tail.

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/content/aip/journal/rsi/79/1/10.1063/1.2828054
2008-01-10
2014-04-21
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Time-resolved scanning system for double reflectance and transmittance fluorescence imaging of diffusive media
http://aip.metastore.ingenta.com/content/aip/journal/rsi/79/1/10.1063/1.2828054
10.1063/1.2828054
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