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Optical trapping and surgery of living yeast cells using a single laser
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10.1063/1.2999542
/content/aip/journal/rsi/79/10/10.1063/1.2999542
http://aip.metastore.ingenta.com/content/aip/journal/rsi/79/10/10.1063/1.2999542
View: Figures

Figures

Image of FIG. 1.
FIG. 1.

Experimental setup for laser trapping and surgery of living yeast cells.

Image of FIG. 2.
FIG. 2.

Sequential bright field images of an optically manipulated single yeast cell using scanning cw Ti:sapphire laser (supplementary movie_01).20 White arrows indicate the beam path.

Image of FIG. 3.
FIG. 3.

Bright field time-lapse images of optically trapped yeast cell growth using cw Ti:sapphire laser at 10 mW of laser power.

Image of FIG. 4.
FIG. 4.

(a) Sequential bright field images of yeast cell wall dissection using femtosecond-pulsed Ti:sapphire laser irradiation. Gray triangles indicate the position of the laser focus (supplementary movie_02).20 (b) Sequential bright field images of yeast's intracellular organelle disruption using femtosecond-pulsed Ti:sapphire laser irradiation. Gray triangles indicate the position of the laser focus (supplementary movie_03).20

Image of FIG. 5.
FIG. 5.

(a) Sequential bright field images of yeast cells with Ti:sapphire laser irradiation. Unfilled and filled triangles indicate the position of the laser focus in cw and femtosecond-pulse operation mode operation, respectively. (b) Number of dissections of yeast cell wall as a function of laser power. Exposure time was fixed to 5 s and 20 cells were irradiated per data point.

Image of FIG. 6.
FIG. 6.

Intracellular organelle extraction and manipulation using the combined technique of optical surgery and trapping. Unfilled and filled triangles indicate the position of the laser focus of the cw and femtosecond-pulsed Ti:sapphire laser, respectively. Black arrows indicate targeted intracellular organelle (supplementary movie_04).20

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/content/aip/journal/rsi/79/10/10.1063/1.2999542
2008-10-20
2014-04-16
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Optical trapping and surgery of living yeast cells using a single laser
http://aip.metastore.ingenta.com/content/aip/journal/rsi/79/10/10.1063/1.2999542
10.1063/1.2999542
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