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Three-dimensional hydrodynamic focusing in a microfluidic Coulter counter
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10.1063/1.2900010
/content/aip/journal/rsi/79/4/10.1063/1.2900010
http://aip.metastore.ingenta.com/content/aip/journal/rsi/79/4/10.1063/1.2900010
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Figures

Image of FIG. 1.
FIG. 1.

(a) Schematic of the three flow cytometer designs built and tested. Dark gray regions are thin (typically ) and light gray regions are tall (typically ). (b) 2D focused flow device 1, showing a ribbon of particle-laden fluids crossing the sensing electrodes. (c) 2D focused flow device 2, which has a tall outlet channel. (d) 3D focused flow in device 3 is caused by stepping the outlet channel back down to the lower level.

Image of FIG. 2.
FIG. 2.

Top-view microscope images of flow, contrasting 2D focusing in device 2 with 3D focusing caused by the step in device 3. In all cases, width of channels is , central flow rate is in the range of , side-stream flow rate , and flow is from left to right. (a) Confocal slice of dye flow in device 2 at a height of above the electrode plane. (b) Confocal slice of focused dye flow in device 3 at the electrode plane. (c) Confocal slice of dye flow in device 3 at above the electrode plane; in contrast with (a), note that the outlet stream is diverted out of focus to the bottom of the channel by undyed fluid from above. (d) Fluorescence micrograph of beads and dye in device 3 during a typical experiment.

Image of FIG. 3.
FIG. 3.

Relative conductivity vs time plots for (a) beads flowing in device 1, (b) beads flowing in device 2, (c) 20 and beads flowing in device 3, and (d) beads flowing in device 3.

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/content/aip/journal/rsi/79/4/10.1063/1.2900010
2008-04-14
2014-04-19
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Three-dimensional hydrodynamic focusing in a microfluidic Coulter counter
http://aip.metastore.ingenta.com/content/aip/journal/rsi/79/4/10.1063/1.2900010
10.1063/1.2900010
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