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Ion thermalization using pressure transients in a quadrupole ion trap coupled to a vacuum matrix-assisted laser desorption ionization source and a reflectron time-of-flight mass analyzer
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10.1063/1.2919881
/content/aip/journal/rsi/79/5/10.1063/1.2919881
http://aip.metastore.ingenta.com/content/aip/journal/rsi/79/5/10.1063/1.2919881

Figures

Image of FIG. 1.
FIG. 1.

AXSIM model of the vacuum MALDI ion source and QIT.

Image of FIG. 2.
FIG. 2.

Schematic diagram of the differentially pumped shroud enclosing the QIT showing two inlet ports for pulsed introduction of gases, additional inlet for controlling background pressure, inlet for the tabulated ion gauge, and pumping outlet.

Image of FIG. 3.
FIG. 3.

Helium ion current transients monitored by an oscilloscope and converted into pressure profiles for a series of electric pulses applied to the solenoid valve.

Image of FIG. 4.
FIG. 4.

Translational energy landscape for injected mass range in the QIT prior to application of the rf voltage. Six ion groups of a particular ratio are highlighted.

Image of FIG. 5.
FIG. 5.

Trapping efficiency as a function of for two helium transients with full-width-at-half-maximum pressures of 1 and . The voltage settings are as follows: rf voltage amplitude of , , dc pulse applied to exit end cap, and rf start time of .

Image of FIG. 6.
FIG. 6.

Positive ion MALDI mass spectrum of a peptide mixture using DHB as a matrix. The intensity scale is linear. (a) Fragmentation is observed for a pressure peak at . (b) Fragmentation suppression is achieved for pressure peak at . ● Spectral lines correspond to bradykinin, ; angiotensin II, ; angiotensin I, ; , ; -acetyl renin at ; ACTH (1–17), ; ACTH (18–39), ▼ Residual fragments.

Image of FIG. 7.
FIG. 7.

Positive ion MALDI mass spectrum of a peptide mixture using CHCA as a matrix. The intensity scale is linear.

Image of FIG. 8.
FIG. 8.

MALDI TOF and MALDI QIT TOF mass spectra of glycogen phosphorylase G tryptic peptide digest using CHCA as a matrix. The intensity scale is linear.

Image of FIG. 9.
FIG. 9.

MALDI QIT TOF mass spectrum of glycogen phosphorylase G tryptic peptide digest using CHCA as a matrix. The intensity scale is linear.

Image of FIG. 10.
FIG. 10.

Radial and axial ion vibrations as a function of time following the application of the rf trapping field. The simulated pressure transient peaks at .

Image of FIG. 11.
FIG. 11.

Radial and axial ion vibrations as a function of time following the application of the rf trapping field. The simulated pressure transient peaks at .

Tables

Generic image for table
Table I.

Peptide mass and corresponding fragment ions generated during ion injection.

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/content/aip/journal/rsi/79/5/10.1063/1.2919881
2008-05-14
2014-04-25
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Ion thermalization using pressure transients in a quadrupole ion trap coupled to a vacuum matrix-assisted laser desorption ionization source and a reflectron time-of-flight mass analyzer
http://aip.metastore.ingenta.com/content/aip/journal/rsi/79/5/10.1063/1.2919881
10.1063/1.2919881
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