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Confocal single molecule fluorescence spectroscopy in ultrahigh vacuum
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10.1063/1.3238505
/content/aip/journal/rsi/80/10/10.1063/1.3238505
http://aip.metastore.ingenta.com/content/aip/journal/rsi/80/10/10.1063/1.3238505
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Figures

Image of FIG. 1.
FIG. 1.

Overview of UHV confocal fluorescence microscope. (A) Port aligner. (B) Load lock and sample heater. (C) Sample deposition chamber with Knudsen cell. (D) Analysis chamber with optical microscope. (E) Rotary wobble stick for locking sample to the retainer. (F) Ion pump. (G) Confocal optics including dichroic, emitter filters, tube lens, and confocal pinhole.

Image of FIG. 2.
FIG. 2.

Close up of microscope. (A) Mounting assembly attaching microscope to 8 in. CF flange. (B) Rotary wobble stick with hex blade for locking sample to the retainer. (C) Reflective microscope objective. (D) Sample retainer with set screw. (E) Sample storage brackets. (F) Piezoelectric stepper for sample focusing. (G) Closed-loop translation stage for sample scanning.

Image of FIG. 3.
FIG. 3.

Optical layout of microscope and light path. multiline laser. F1: neutral density filter bank. PBC: polarizing beamsplitter cube. HWP: halfwave plate. F2: exciter filter. PBS: pellicle beamsplitter. DBS: dichroic beamsplitter. QVP: quartz viewport. Obj: microscope objective. F3 and F4: emitter filters. L1: tube lens. PH: confocal pinhole. L2: relay lens. APD: avalanche photodiode. DAQ: data acquisition.

Image of FIG. 4.
FIG. 4.

confocal fluorescence microscopy image of a 20-nm-diameter dye-stained bead excited at 488 nm, and lineouts showing near-diffraction-limited performance. and . The arrow indicates the polarization of the laser field in the plane of the sample.

Image of FIG. 5.
FIG. 5.

images of -PTCDI molecules prepared and recorded in UHV setup at 488 nm. A) PTCDI on ozone-cleaned glass cover slip. B) PTCDI on 45 Å (111)/GaN (0001). Most fluorescence spots correspond to single PTCDI molecules except for the brightest feature in each image which is likely due to a cluster of molecules.

Image of FIG. 6.
FIG. 6.

Fluorescence trajectories (black) of a single -PTCDI molecule, excited at 488 nm, together with CPD analysis (red) on (a) ozone-cleaned glass cover slip, (b) (0001), and (c) 45 Å . Three fluorescence intensities are observed: bright, dark, and shutter closed. Note the very different time axes for the three trajectories, with the molecule on never permanently switching to a dark state over the observation period.

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/content/aip/journal/rsi/80/10/10.1063/1.3238505
2009-10-06
2014-04-21
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Confocal single molecule fluorescence spectroscopy in ultrahigh vacuum
http://aip.metastore.ingenta.com/content/aip/journal/rsi/80/10/10.1063/1.3238505
10.1063/1.3238505
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