Chemical structure of LDS 798.
Absorption and fluorescence spectra of LDS 798 in EtOH (top) and PBS (bottom).
Fluorescence intensity decays of LDS 798 in (a) EtOH and (b) PBS 7.4.
Fluorescence intensity decays of LDS 798 in PBS, 7.4, excited at 470 nm, observed at 770 nm.
APD (Perkin-Elmer SPCM-AQR-14) responses to the pulsed 470 nm excitation. (a) Scattering from colloidal silica observed at 470 nm. (b) Fluorescence signal from LDS 798 in PBS, 7.4, observed at longer wavelengths .
Fluorescence intensity decay of Rh800 in EtOH, detected with Perkin-Elmer SPCM-AQR-14 detector. IRF is approximated by the temporal profile of scattered 470 nm excitation light. The single exponential model (smooth curve) does not match the experimental data.
Fluorescence intensity decay of Rh800 in EtOH, the same as in Fig. 6. IRF is approximated by the fast fluorescence of LDS 798 in PBS solution. A single exponential reconvolution fit successfully describes the data and yields the correct lifetime.
Fluorescence intensity decay of PY1 in EtOH together with the temporal profile of scattered 470 nm excitation light and an unsatisfactory single exponential fit. There are strong, systematic deviations in the distribution of residuals (bottom panel).
Fluorescence intensity decay of PY1 in EtOH (the same as in Fig. 8). The fast fluorescence of LDS 798 in the PBS solution is included as an IRF approximation. Correct decay parameters are recovered and the quality of the fit is acceptable.
The measured lifetimes of LDS 798, Rh800, and PY1 in EtOH and PBS using different detectors and IRFs.
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