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Instrument response standard in time-resolved fluorescence
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10.1063/1.3095677
/content/aip/journal/rsi/80/3/10.1063/1.3095677
http://aip.metastore.ingenta.com/content/aip/journal/rsi/80/3/10.1063/1.3095677

Figures

Image of FIG. 1.
FIG. 1.

Chemical structure of LDS 798.

Image of FIG. 2.
FIG. 2.

Absorption and fluorescence spectra of LDS 798 in EtOH (top) and PBS (bottom).

Image of FIG. 3.
FIG. 3.

Fluorescence intensity decays of LDS 798 in (a) EtOH and (b) PBS 7.4.

Image of FIG. 4.
FIG. 4.

Fluorescence intensity decays of LDS 798 in PBS, 7.4, excited at 470 nm, observed at 770 nm.

Image of FIG. 5.
FIG. 5.

APD (Perkin-Elmer SPCM-AQR-14) responses to the pulsed 470 nm excitation. (a) Scattering from colloidal silica observed at 470 nm. (b) Fluorescence signal from LDS 798 in PBS, 7.4, observed at longer wavelengths .

Image of FIG. 6.
FIG. 6.

Fluorescence intensity decay of Rh800 in EtOH, detected with Perkin-Elmer SPCM-AQR-14 detector. IRF is approximated by the temporal profile of scattered 470 nm excitation light. The single exponential model (smooth curve) does not match the experimental data.

Image of FIG. 7.
FIG. 7.

Fluorescence intensity decay of Rh800 in EtOH, the same as in Fig. 6. IRF is approximated by the fast fluorescence of LDS 798 in PBS solution. A single exponential reconvolution fit successfully describes the data and yields the correct lifetime.

Image of FIG. 8.
FIG. 8.

Fluorescence intensity decay of PY1 in EtOH together with the temporal profile of scattered 470 nm excitation light and an unsatisfactory single exponential fit. There are strong, systematic deviations in the distribution of residuals (bottom panel).

Image of FIG. 9.
FIG. 9.

Fluorescence intensity decay of PY1 in EtOH (the same as in Fig. 8). The fast fluorescence of LDS 798 in the PBS solution is included as an IRF approximation. Correct decay parameters are recovered and the quality of the fit is acceptable.

Tables

Generic image for table
Table I.

The measured lifetimes of LDS 798, Rh800, and PY1 in EtOH and PBS using different detectors and IRFs.

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/content/aip/journal/rsi/80/3/10.1063/1.3095677
2009-03-17
2014-04-18
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Instrument response standard in time-resolved fluorescence
http://aip.metastore.ingenta.com/content/aip/journal/rsi/80/3/10.1063/1.3095677
10.1063/1.3095677
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