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Construction of an integrated Raman- and angular-scattering microscope
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View: Figures


Image of FIG. 1.
FIG. 1.

System diagram: light (gray) paths indicate laser illumination paths, darker (green) paths indicate bright field illumination and collection paths, and darkest (red) paths indicate laser collection paths. Abbreviations: beam splitter, beam sampler, notch filter, plate, laser source, density filter, , , plate, , , and prism.

Image of FIG. 2.
FIG. 2.

IRAM measurements of Saccharomyces cerevisiae. (a) Bright field image of the measured yeast cell. Scale bar indicates . (b) Raman spectrum from the yeast cell shown in (a). (c) Elastic scattering pattern from the yeast cell [color scale runs from blue (low) to red (high)]. (d) Azimuthally averaged data from (c) (black dots) and associated best fit using a coated sphere model (solid blue line), with extracted core and coat diameters of 6.06 and , respectively.

Image of FIG. 3.
FIG. 3.

IRAM measurements of Streptococcus pneumoniae. (a) Elastic scattering pattern from a population of bacteria [color scale runs from blue (low) to red (high)]. (b) One of four azimuthally averaged bins of data from (a), representing an average of angles (black dots). Also shown is the best fit to the data using a single log-normal population of scatterers (solid blue line). (c) Predicted population of bacteria sizes, showing a mean of 727 nm and a standard deviation of 52 nm. (d) Raman spectrum of a single bacterium.


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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Construction of an integrated Raman- and angular-scattering microscope