1887
banner image
No data available.
Please log in to see this content.
You have no subscription access to this content.
No metrics data to plot.
The attempt to load metrics for this article has failed.
The attempt to plot a graph for these metrics has failed.
Design and validation of a novel bioreactor principle to combine online micro-computed tomography monitoring and mechanical loading in bone tissue engineering
Rent:
Rent this article for
USD
10.1063/1.3284787
/content/aip/journal/rsi/81/1/10.1063/1.3284787
http://aip.metastore.ingenta.com/content/aip/journal/rsi/81/1/10.1063/1.3284787

Figures

Image of FIG. 1.
FIG. 1.

Scheme of the feedback controlled system, as proposed for future studies. Additional feedback parameters may be obtained from medium analysis (e.g., level in the medium or lactate consumption). Additional control parameters may be growth factors, which are added to the medium or remaining culture time.

Image of FIG. 2.
FIG. 2.

Scheme of the MSU including culture chamber and control unit.

Image of FIG. 3.
FIG. 3.

Photographs of the MSU and the culture chambers. One culture chamber as it is mounted in the MSU inside a sterile laminar flow bench (a). Close up of the chamber in the MSU (b). Twelve culture chambers inside an incubator and connected to medium circulation tubes (c).

Image of FIG. 4.
FIG. 4.

Displacement validation: (a) Bode plot illustrating the amplification of the measured displacement amplitude in relation to the commanded displacement amplitude for commanded amplitudes of 80 and . (b) THD as a function of the commanded sine wave’s frequency for commanded amplitudes of 80 and . (c) Graph illustrating the measured displacement at 10 Hz with an amplitude of .

Image of FIG. 5.
FIG. 5.

Force-displacement plots for two different springs as measured on a Zwick material testing machine and on the custom-made MSU. The axis is without a scale because the curves obtained from the two different machines were shifted in direction to each other to illustrate the parallel force-displacement measurement. The two noisier curves ( and ) were measured using the custom-made MSU.

Image of FIG. 6.
FIG. 6.

3D images of human bone biopsy specimen as measured by using (a) the original scanning vial and (b) the custom-made culture chamber.

Tables

Generic image for table
Table I.

Commanded and measured displacement to test the displacement accuracy and precision.

Generic image for table
Table II.

Morphological parameters of bone biopsy specimen upon scanning as measured in original scanning vial vs custom-made culture chamber (-value as determined by Student’s t-test).

Loading

Article metrics loading...

/content/aip/journal/rsi/81/1/10.1063/1.3284787
2010-01-25
2014-04-20
Loading

Full text loading...

This is a required field
Please enter a valid email address
752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Design and validation of a novel bioreactor principle to combine online micro-computed tomography monitoring and mechanical loading in bone tissue engineering
http://aip.metastore.ingenta.com/content/aip/journal/rsi/81/1/10.1063/1.3284787
10.1063/1.3284787
SEARCH_EXPAND_ITEM