(a) Photograph of the McCAEPs. The heated microchip platform on the top of the instrument uses three leveling screws to adjust parallelism between the detection plane and the rotating objective focal plane. Pressure/vacuum output connections (four groups of eight) on the top edges of the side support plates are attached with 1/8 in. tubing to the chip manifold pneumatic connections. (b) Exploded view of the instrument. The major structural components of the instrument include the heated microchip platform, a main support plate on which all the optical and pneumatic components are located, two side support plates, and a bottom plate. (c) View of the open instrument with the bottom cover hinged down.
(a) Schematic of the four-color confocal detection system showing the relative positions of the laser, dichroic beam splitter, hollow shaft motor, rhomb assembly with objective, with a rotary objective, confocal assembly, and PMT. (b) Exploded view of the 96-lane manifold. The electrode ring assembly consists of a circuit board with Pt electrodes sandwiched in a Bakelite holder and the connections to the sample electrodes. The electrodes are connected with wires to the high voltage output ports on the right side support plate (see in Fig. 1). Two sets of drip needles connected to a peristaltic pump system maintain buffer levels in the moat rings on the microchip during electrophoresis at .
(a) The signal to noise as a function of fluorescein concentration. The limit of detection of the system is determined as with a S/N of 2. (b) Representative PowerPlex 16 profiles amplified from 1 ng of 9947A female standard DNA obtained using the 96-lane microchip on the instrument. Alleles in penta E, penta D, and FGA are zoomed for illustration.
(a) Thermal cycling amplification profile measured from an integrated PCR-CE microchip. Solid line shows the measured temperature from the RTD and dash line is the set temperature. Temperature ramp rates were for heating and for cooling. (b) DNA profiles amplified from 100 copies of 9948 male standard DNA using the integrated PCR-CE microsystem.
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