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Combined system of fluorescence diffuse optical tomography and microcomputed tomography for small animal imaging
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10.1063/1.3422252
/content/aip/journal/rsi/81/5/10.1063/1.3422252
http://aip.metastore.ingenta.com/content/aip/journal/rsi/81/5/10.1063/1.3422252

Figures

Image of FIG. 1.
FIG. 1.

Scheme of the system. The micro-CT and the fDOT are mounted orthogonally to each other. The chamber holding animals is fixed onto the rotation stage and rotates with the rotation stage, while micro-CT scans are performed. The translation stage scans in a horizontal direction. Data acquisition and scanning are under the control of the computer. CCD is charge coupled device.

Image of FIG. 2.
FIG. 2.

Presampled MTF of the micro-CT and the FPD. The LSF of the micro-CT and the FPD are measured with a tungsten wire with a diameter of and a stainless steel slant edge. The presampled MTF is calculated by taking the Fourier transform of the LSF.

Image of FIG. 3.
FIG. 3.

Control protocol of the system. N represents the number of projection image captured in the scans of the micro-CT. M indicates the number of scanning points in the fDOT scans. Elapsed times for data transmission and FPD rotation stage movement are both 80 ms. A CCD camera captures the projection image of incident light and fluorescence at one position. The filter wheel changes the position of the CCD camera.

Image of FIG. 4.
FIG. 4.

Demonstration of the resolution of fDOT. (a) and (d) are the schemes of the phantom. The distances of the glass tubes with Dir-BOA sealing with glue are 2 and 3 mm, respectively. Glass tubes were immersed in the intralipid. (b) and (e) are the transaxial images of the phantom at the position shown by the dotted line in (a) and (d). (c) and (f) are the amplitude profiles from (b) and (e), indicated by black dotted lines.

Image of FIG. 5.
FIG. 5.

Estimate of the quantification accuracy of the fDOT system. (a) Reconstructed raw data at different concentrations of DiR-BOA, analyzed with linear regression (data for 50 nM are not included in regression). (b)–(g) Transaxial images of reconstructed data of the phantom with concentrations from 50 to 500 nM. The position of reconstruction is indicated by the dotted line in (h), which is the scheme of the phantom.

Image of FIG. 6.
FIG. 6.

Merging the results of the glass tube images with the combined system. (a) Transaxial image of the glass tube imaging from the fDOT system. (b) Amplitude profile of the dotted lines in (a). (c) Transaxial image of the glass tube from the micro-CT system. (d) Amplitude profile of the dotted lines in (c). (e) Image merging of (a) and (c) with a non-image-based method. (f) Amplitude profile of the dotted lines in (e). (g) Result of coronal image merging at the position indicated by the dotted line in (e). (h) Result of sagittal image merging at the position indicated by the dotted line in (e).The horizontal dotted lines in (g) and (h) indicate the positions of the transaxial images in (a), (c), and (e).

Image of FIG. 7.
FIG. 7.

(a) Reconstructed transaxial image of the phantom. Voxel size of the reconstruction image is . (b) Profile of the location is indicated by the white line in (a).

Image of FIG. 8.
FIG. 8.

Image of a euthanized nude mouse with optical marker using the combined system. (a) X-ray projection image captured during scanning. (b) Transaxial image in the micro-CT. The position is indicated by the black line in (a). The image of the chamber in the reconstructed image is removed automatically. Gt, glass tube; Li, liver; Lu, Lung; Ri, rib; Ve, vertebrae. (c) Transaxial image in the fDOT; the position is indicated by the black line in (a). (d) Image merging of the micro-CT and the fDOT images.

Tables

Generic image for table
Table I.

Main features of the flat panel detector.

Generic image for table
Table II.

Main features of the CCD camera.

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/content/aip/journal/rsi/81/5/10.1063/1.3422252
2010-05-25
2014-04-21
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752b84549af89a08dbdd7fdb8b9568b5 journal.articlezxybnytfddd
Scitation: Combined system of fluorescence diffuse optical tomography and microcomputed tomography for small animal imaging
http://aip.metastore.ingenta.com/content/aip/journal/rsi/81/5/10.1063/1.3422252
10.1063/1.3422252
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