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A thermal study of cellular motility by optical time-resolved correlation
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Image of FIG. 1.
FIG. 1.

Experimental setup. A beam of a He-Ne laser is optically driven to a chamber that contains a sample holder. This sample holder has six small holes that can be filled with the sample. Light passes through each one of these receptacles. The scattering pattern is recorded by a digital camera for image correlation analysis. The sample temperature is controlled.

Image of FIG. 2.
FIG. 2.

Schematic drawing to show the procedure of obtaining the frames. Note that all measurements were taken in a constant rate of one frame per second.

Image of FIG. 3.
FIG. 3.

A schematic example of the image processing. (a) The first frame at time t 0 is compared with each consecutive frame taken at time t i (i goes from 1 to 180 s). (b) A square of 100 × 100 pixels is taken from the center of each frame as an intensity matrix to be processed by Eq. (1).

Image of FIG. 4.
FIG. 4.

Autocorrelation curves showing motility boundaries. A stretched exponential is fitted to obtain the CMP by Eq. (2).

Image of FIG. 5.
FIG. 5.

Temporal development of the autocorrelation showed by sperm sampled during 6 h. Comparison between (a) 37 °C and (b) 10 °C.

Image of FIG. 6.
FIG. 6.

(a) Temporal development of the CMP showed for different temperatures. The inset shows a zoom in order to appreciate the lower temperatures. (b) Prevalence of sperm motility depending on temperature, represented by the inverse of the area under the curve of the temporal development of CMP.

Image of FIG. 7.
FIG. 7.

A thermal reversibility behavior in sperm motility is observed when performing a temperature drop from 37 °C to 10 °C. The achieved motility is compared with the thermal behavior of the control sample at 10 °C obtained from previous analysis.


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Scitation: A thermal study of cellular motility by optical time-resolved correlation