Experimental setup employed in this study. (a) Flow chart showing each one of the system components. (b) Image of the equipment employed in the laboratory: 1. Computer interface to set the signal parameters. 2. Stimulation system LM3S6965. 3. Signal conditioning. 4. Carbon-based microfluidic device for particle manipulation. 5. Inverted microscope for microfluidics SVM 340. 6. Experimental results as images or video recordings.
Sample signals of the stimulation system: (a) Single sawtooth, (b) single sine, (c) single triangle, (d) sawtooth over sawtooth, (e) triangle over sine, (f) triangle over sawtooth.
(a) Image showing the device fabricated on piece of a silicon wafer. (b) Magnification of a section of the microchannel showing the electrode leads and three-dimensional carbon electrodes. (c) Schematic representation of a section of the microchannel showing the three-dimensional carbon electrode posts; negative electrodes are shown in black on the dark blue electrode leads and positive electrodes are shown in white on the light blue electrode leads. (d) Magnitude of the dielectrophoretic force exerted on 10-μm inert particles and 5-μm yeast cells within the microchannel as specified by the location of the red line depicted in (c).
(a) Plot of the magnitude of f CM for yeast cells and polystyrene particles as function of frequency. At this frequency range (<100 kHz) clear difference between f CM values make separations feasible for the mixtures of microparticles and cells. (b) Image of the particles and cells distributed randomly in the microchannel at 0V: polystyrene beads marked by an orange rectangle are larger in size than the yeast cells marked by a yellow circle. (c) DEP response of the cells and polystyrene beads at 5 Vpp at a frequency of 28 kHz: cells exhibit positive DEP since they are attracted to the electrodes and polystyrene beads exhibit negative DEP as illustrated by orange rectangles.
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