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A high-speed magnetic tweezer beyond 10,000 frames per second
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/content/aip/journal/rsi/84/4/10.1063/1.4802678
2013-04-29
2014-08-21

Abstract

The magnetic tweezer is a single-molecule instrument that can apply a constant force to a biomolecule over a range of extensions, and is therefore an ideal tool to study biomolecules and their interactions. However, the video-based tracking inherent to most magnetic single-molecule instruments has traditionally limited the instrumental resolution to a few nanometers, above the length scale of single DNA base-pairs. Here we have introduced superluminescent diode illumination and high-speed camera detection to the magnetic tweezer, with graphics processing unit-accelerated particle tracking for high-speed analysis of video files. We have demonstrated the ability of the high-speed magnetic tweezer to resolve particle position to within 1 Å at 100 Hz, and to measure the extension of a 1566 bp DNA with 1 nm precision at 100 Hz in the presence of thermal noise.

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Scitation: A high-speed magnetic tweezer beyond 10,000 frames per second
http://aip.metastore.ingenta.com/content/aip/journal/rsi/84/4/10.1063/1.4802678
10.1063/1.4802678
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